The taxonomic status of a Gram-negative-staining bacterium, isolated from the faeces of a seal, was investigated using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that the novel isolate formed a distinct phyletic line within the genus , displaying >3.3 % sequence divergence with other known species. The generic assignment was confirmed by chemotaxonomic data, which revealed a fatty acid profile that included straight-chain saturated, unsaturated and 3-hydroxylated fatty acids, with C 9 as the major fatty acid. A ubiquinone with eight isoprene units (Q-8) was the predominant respiratory quinone and spermidine was the predominant polyamine. The novel isolate was distinguished from other members of the genus by using a set of phenotypic properties. On the basis of phenotypic and phylogenetic considerations, it is proposed that the new isolate represents a novel species, for which the name sp. nov. is proposed. Strain IMMIB L-1110 (=DSM 21542=CCUG 56590) is the type strain.


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vol. , part 8, pp. 2049 - 2053

Phenotypic characteristics of strain IMMIB L-1110 and other recognized species.

Cellular fatty acid composition (%) of strain IMMIB L-1110 and other recognized species.

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