1887

International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 8

sp. nov., isolated from a wound obtained from a dog bite Free

Abstract

A non-lipophilic, coryneform bacterium, isolated from a patient's wound obtained from a dog bite, was characterized by phenotypic, chemotaxonomic and molecular genetic methods. Chemotaxonomic features suggested assignment of the unknown bacterium to the genus . The isolate exhibited the following peculiar features which made it possible to differentiate it phenotypically from all other medically relevant corynebacteria: older colonies exhibited a ‘spoke-wheel’ macroscopic morphology, colonies were strongly adherent to blood agar and the strain did not have pyrazinamidase activity, but was positive for -galactosidase. 16S rRNA gene sequencing showed that the closest phylogenetic relative exhibited more than 3.9 % divergence from the unknown isolate. Based on phenotypic and molecular genetic data, it is proposed that the isolate should be classified as a representative of a novel species, sp. nov., with strain 1045 (=CCUG 56874 =DSM 45254) as the type strain.

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008672-0
2009-08-01
2021-03-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/8/2054.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008672-0&mimeType=html&fmt=ahah

References

  1. Beck, M., Frodl, R. & Funke, G. ( 2008; ). Comprehensive study of strains previously designated Streptococcus bovis consecutively isolated from human blood cultures and emended description of Streptococcus gallolyticus and Streptococcus infantarius subsp. coli. J Clin Microbiol 46, 2966–2972.[CrossRef]
    [Google Scholar]
  2. CLSI ( 2006; ). Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, document M45-A. Wayne, PA: Clinical and Laboratory Standards Institute.
  3. Collins, M. D., Bernard, K. A., Hutson, R. A., Sjödén, B., Nyberg, A. & Falsen, E. ( 1999a; ). Corynebacterium sundsvallense sp. nov., from human clinical specimens. Int J Syst Bacteriol 49, 361–366.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Hoyles, L., Lawson, P. A., Falsen, E., Robson, R. L. & Foster, G. ( 1999b; ). Phenotypic and phylogenetic characterization of a new Corynebacterium species from dogs: description of Corynebacterium auriscanis sp. nov. J Clin Microbiol 37, 3443–3447.
     [Google Scholar]
  5. Collins, M. D., Hoyles, L., Hutson, R. A., Foster, G. & Falsen, E. ( 2001; ). Corynebacterium testudinoris sp. nov., from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat. Int J Syst Evol Microbiol 51, 1349–1352.
     [Google Scholar]
  6. Collins, M. D., Hoyles, L., Foster, G. & Falsen, E. ( 2004; ). Corynebacterium caspium sp. nov., from a Caspian seal (Phoca caspica). Int J Syst Evol Microbiol 54, 925–928.[CrossRef]
    [Google Scholar]
  7. Fernández-Garayzábal, J. F., Vela, A. I., Egido, R., Hutson, R. A., Lanzarot, M. P., Fernández-García, M. & Collins, M. D. ( 2004; ). Corynebacterium ciconiae sp. nov., isolated from the trachea of black storks (Ciconia nigra). Int J Syst Evol Microbiol 54, 2191–2195.[CrossRef]
    [Google Scholar]
  8. Funke, G. & Bernard, K. A. ( 2007; ). Coryneform gram-positive rods. In Manual of Clinical Microbiology, 9th edn, pp. 485–514. Edited by P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry & M. A. Pfaller. Washington, DC: American Society for Microbiology.
  9. Funke, G., Martinetti Lucchini, G., Pfyffer, G. E., Marchiani, M. & von Graevenitz, A. ( 1993; ). Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens. J Clin Microbiol 31, 2907–2912.
     [Google Scholar]
  10. Funke, G., Bernard, K. A., Bucher, C., Pfyffer, G. E. & Collins, M. D. ( 1995; ). Corynebacterium glucuronolyticum sp. nov. isolated from male patients with genitourinary infections. Med Microbiol Lett 4, 204–215.
     [Google Scholar]
  11. Funke, G., Lawson, P. A., Bernard, K. A. & Collins, M. D. ( 1996; ). Most Corynebacterium xerosis strains identified in the routine clinical laboratory correspond to Corynebacterium amycolatum. J Clin Microbiol 34, 1124–1128.
     [Google Scholar]
  12. Lartigue, M.-F., Monnet, X., Le Flèche, A., Grimont, P. A. D., Benet, J.-J., Durrbach, A., Fabre, M. & Nordmann, P. ( 2005; ). Corynebacterium ulcerans in an immunocompromised patient with diphtheria and her dog. J Clin Microbiol 43, 999–1001.[CrossRef]
    [Google Scholar]
  13. Nakao, H. & Popovic, T. ( 1997; ). Development of a direct PCR assay for detection of the diphtheria toxin gene. J Clin Microbiol 35, 1651–1655.
     [Google Scholar]
  14. Rassoulian Barrett, S. L., Cookson, B. T., Carlson, L. C., Bernard, K. A. & Coyle, M. B. ( 2001; ). Diversity within reference strains of Corynebacterium matruchotii includes Corynebacterium durum and a novel organism. J Clin Microbiol 39, 943–948.[CrossRef]
    [Google Scholar]
  15. Riegel, P., Heller, R., Prevost, G., Jehl, F. & Monteil, H. ( 1997; ). Corynebacterium durum sp. nov., from human clinical specimens. Int J Syst Bacteriol 47, 1107–1111.[CrossRef]
    [Google Scholar]
  16. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  17. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  18. Yassin, A. F. ( 2007; ). Corynebacterium ureicelerivorans sp. nov., a lipophilic bacterium isolated from blood culture. Int J Syst Evol Microbiol 57, 1200–1203.[CrossRef]
    [Google Scholar]
  19. Yassin, A. F., Steiner, U. & Ludwig, W. ( 2002; ). Corynebacterium appendicis sp. nov. Int J Syst Evol Microbiol 52, 1165–1169.[CrossRef]
    [Google Scholar]
  20. Zimmermann, O., Spröer, C., Kroppenstedt, R. M., Fuchs, E., Köchel, H. G. & Funke, G. ( 1998; ). Corynebacterium thomssenii sp. nov., a Corynebacterium with N-acetyl-β-glucosaminidase activity from human clinical specimens. Int J Syst Bacteriol 48, 489–494.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008672-0
Loading
Corynebacterium freiburgense sp. nov., isolated from a wound obtained from a dog bite
Int J Syst Evol Microbiol 59, 2054 (2009); https://doi.org/10.1099/ijs.0.008672-0
/content/journal/ijsem/10.1099/ijs.0.008672-0
/content/journal/ijsem/10.1099/ijs.0.008672-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error