A novel actinomycete, strain OFN S31, was isolated from a complex biofilm in the Altamira Cave, Spain. A polyphasic study was carried out to clarify the taxonomic position of this strain. Phylogenetic analysis with 16S rRNA gene sequences of representatives of the genera , , , , , , , , , and indicated that strain OFN S31 formed a distinct taxon in the 16S rRNA gene tree that was more closely associated with the clade. The type strain of was the closest relative of strain OFN S31 (95.6 % similarity). The cell wall contained -diaminopimelic acid, arabinose and galactose, which are characteristic components of cell-wall chemotype IV of actinomycetes. The sugars of the peptidoglycan were acetylated. The polar lipid pattern was composed of phosphatidylinositol, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Strain OFN S31 is characterized by the absence of mycelium and mycolic acids. Strain OFN S31 had MK-8 as the major menaquinone. The DNA G+C content was 49.3 mol%, the lowest found among all taxa included in the suborder . Based on morphological, chemotaxonomic, phenotypic and genetic characteristics, strain OFN S31 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is strain OFN S31 (=CIP 109864 =DSM 45258).


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