1887

Abstract

Novel aerobic, Gram-negative bacteria with DNA G+C contents below 50 mol% were isolated from the culturable microbiota associated with the Mediterranean seagrass . 16S rRNA gene sequence analyses revealed that they belong to the genus . Strain IVIA-Po-186 is a strain of the species , showing 99.77 % 16S rRNA gene sequence similarity with the type strain, MMB-1, and sharing all phenotypic characteristics studied. This is the first description of this species forming part of the microbiota of a marine plant. A second strain, designated IVIA-Po-101, was closely related to based on phylogenetic studies. However, it differed in characteristics such as melanin synthesis and tyrosinase, laccase and antimicrobial activities. In addition, strain IVIA-Po-101 was auxotrophic and unable to use acetate. IVIA-Po-101 shared 97.86 % 16S rRNA gene sequence similarity with MMB-1, but the level of DNA–DNA relatedness between the two strains was only 10.3 %. On the basis of these data, strain IVIA-Po-101 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IVIA-Po-101 (=CECT 7378 =NCIMB 14432). A third novel strain, IVIA-Po-185, was phylogenetically distant from all recognized species. It shared the highest 16S rRNA gene sequence similarity (97.4 %) with the type strain of , but the level of DNA–DNA relatedness between the two strains was only 14.5 %. A differential chemotaxonomic marker of this strain in the genus is the presence of the fatty acid C cyclo. Strain IVIA-Po-185 is thus considered to represent a second novel species of the genus, for which the name sp. nov. is proposed. The type strain is IVIA-Po-185 (=CECT 7375 =NCIMB 14435). An emended description of the genus is given based on the description of these two novel species, as well as other species described after the original description of the genus.

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2010-01-01
2020-01-22
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