1887

Abstract

An orange-coloured actinomycete, designated strain RP-B16, was isolated from a rhizosphere soil. Cells of strain RP-B16 were strictly aerobic, Gram-positive-staining, oxidase-negative, catalase-positive, motile cocci. A phylogenetic tree, based on 16S rRNA gene sequences, showed that members of the genus were divided into two independent clusters and that strain RP-B16 formed a coherent clade with the type strains of (99.5 % sequence similarity) and (99.1 %). Levels of 16S rRNA gene sequence similarity between strain RP-B16 and KST3-3 and ‘’ T2A-S27 were lower than 97.0 %. Chemotaxonomic characteristics were typical of the genus in having -diaminopimelic acid as the diagnostic diamino acid, MK-9(H) as the predominant menaquinone, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids, anteiso-C as the predominant fatty acid and a DNA G+C content of 73.8 mol%. Strain RP-B16 showed levels of DNA–DNA relatedness of 34.6–37.3 % to DSM 7487 and 31.2–32.7 % to DSM 14245. On the basis of phenotypic features and DNA–DNA hybridization data, strain RP-B16 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RP-B16 (=KCTC 19366 =DSM 19711).

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2009-09-01
2021-08-05
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