1887

Abstract

An orange-coloured actinomycete, designated strain RP-B16, was isolated from a rhizosphere soil. Cells of strain RP-B16 were strictly aerobic, Gram-positive-staining, oxidase-negative, catalase-positive, motile cocci. A phylogenetic tree, based on 16S rRNA gene sequences, showed that members of the genus were divided into two independent clusters and that strain RP-B16 formed a coherent clade with the type strains of (99.5 % sequence similarity) and (99.1 %). Levels of 16S rRNA gene sequence similarity between strain RP-B16 and KST3-3 and ‘’ T2A-S27 were lower than 97.0 %. Chemotaxonomic characteristics were typical of the genus in having -diaminopimelic acid as the diagnostic diamino acid, MK-9(H) as the predominant menaquinone, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids, anteiso-C as the predominant fatty acid and a DNA G+C content of 73.8 mol%. Strain RP-B16 showed levels of DNA–DNA relatedness of 34.6–37.3 % to DSM 7487 and 31.2–32.7 % to DSM 14245. On the basis of phenotypic features and DNA–DNA hybridization data, strain RP-B16 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RP-B16 (=KCTC 19366 =DSM 19711).

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2009-09-01
2019-12-13
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References

  1. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridisation from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  3. Duangmal, K., Thamchaipenet, A., Ara, I., Matsumoto, A. & Takahashi, Y. ( 2008; ). Kineococcus gynurae sp. nov., isolated from a Thai medicinal plant. Int J Syst Evol Microbiol 58, 2439–2442.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  7. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  9. Lee, S. D. ( 2006; ). Kineococcus marinus sp. nov., isolated from marine sediment of the coast of Jeju, Korea. Int J Syst Evol Microbiol 56, 1279–1283.[CrossRef]
    [Google Scholar]
  10. Lee, S. D. & Lee, D. W. ( 2007; ). Lapillicoccus jejuensis gen. nov., sp. nov., a novel actinobacterium of the family Intrasporangiaceae, isolated from stone. Int J Syst Evol Microbiol 57, 2794–2798.[CrossRef]
    [Google Scholar]
  11. MacFaddin, J. F. ( 1980; ). Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Minnikin, D. E., Patel, P. V., Alshamaony, L. & Goodfellow, M. ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27, 104–117.[CrossRef]
    [Google Scholar]
  14. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  15. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  16. Phillips, R. W., Wiegel, J., Berry, C. J., Fliermans, C., Peacock, A. D., White, D. C. & Shimkets, L. J. ( 2002; ). Kineococcus radiotolerans sp. nov., a radiation-resistant, Gram-positive bacterium. Int J Syst Evol Microbiol 52, 933–938.[CrossRef]
    [Google Scholar]
  17. Saddler, G. S., Tavecchia, P., Lociuro, S., Zanol, M., Colombo, E. & Selva, E. ( 1991; ). Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14, 185–191.[CrossRef]
    [Google Scholar]
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Seo, J. P. & Lee, S. D. ( 2006; ). Nocardia harenae sp. nov., an actinomycete isolated from beach sand. Int J Syst Evol Microbiol 56, 2203–2207.[CrossRef]
    [Google Scholar]
  20. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  21. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  24. Yokota, A., Tamura, T., Nishii, T. & Hasegawa, T. ( 1993; ). Kineococcus aurantiacus gen. nov., a new aerobic, gram-positive, motile coccus with meso-diaminopimelic acid and arabinogalactan in the cell wall. Int J Syst Bacteriol 43, 52–57.[CrossRef]
    [Google Scholar]
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