1887

Abstract

An aerobic, Gram-stain-negative, rod-shaped bacterial isolate, strain NH36A, was isolated from a sandy sediment sample from the South China Sea. Colonies of the isolate were dark orange on M2 agar. Optimal growth was observed at pH 7.0–8.5, 30 °C and in the presence of 0.5–4.0 % (w/v) NaCl. The major fatty acids were C, iso-C, anteiso-C, iso-C, iso-C 3-OH, iso-C 3-OH and summed feature 3 (comprising iso-C 2-OH and/or C 7). The DNA G+C content was 38.9 mol%. 16S rRNA gene sequence analysis revealed that strain NH36A was most closely related to members of the genus , exhibiting 94.3–96.2 % sequence similarity to the type strains of species. On the basis of phenotypic, chemotaxonomic and phylogenetic data, this organism should be classified as a representative of a novel species in the genus . The name sp. nov. is proposed and the type strain is NH36A (=LMG 24842=CCTCC AB 208315=MCCC 1A04137).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008573-0
2010-01-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/1/78.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008573-0&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) ( 1995; ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: John Wiley.
  2. Christakis, G. B., Perlorentzou, S. P., Chalkiopoulou, I., Athanasiou, A. & Legakis, N. J. ( 2005; ). Chryseobacterium indologenes non-catheter-related bacteremia in a patient with a solid tumor. J Clin Microbiol 43, 2021–2023.[CrossRef]
    [Google Scholar]
  3. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  4. Ivanova, E. P., Kiprianova, E. A., Mikhailov, V. V., Levanova, G. F., Garagulya, A. D., Gorshkova, N. M., Vysotskii, M. V., Nicolau, D. V., Yumoto, N. & other authors ( 1998; ). Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48, 247–256.[CrossRef]
    [Google Scholar]
  5. Ivanova, E. P., Nedashkovskaya, O. I., Chun, J., Lysenko, A. M., Frolova, G. M., Svetashev, V. I., Vysotskii, M. V., Mikhailov, V. V., Huq, A. & Colwell, R. R. ( 2001; ). Arenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 51, 1987–1995.[CrossRef]
    [Google Scholar]
  6. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  7. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  8. Nedashkovskaya, O. I., Suzuki, M., Vysotskii, M. V. & Mikhailov, V. V. ( 2003; ). Arenibacter troitsensis sp. nov., isolated from marine bottom sediment. Int J Syst Evol Microbiol 53, 1287–1290.[CrossRef]
    [Google Scholar]
  9. Nedashkovskaya, O. I., Kim, S. B., Han, S. K., Lysenko, A. M., Mikhailov, V. V. & Bae, K. S. ( 2004; ). Arenibacter certesii sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 54, 1173–1176.[CrossRef]
    [Google Scholar]
  10. Nedashkovskaya, O. I., Vancanneyt, M., Cleenwerck, I., Snauwaert, C., Kim, S. B., Lysenko, A. M., Shevchenko, L. S., Lee, K. H., Park, M. S. & other authors ( 2006; ). Arenibacter palladensis sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata, and emended description of the genus Arenibacter. Int J Syst Evol Microbiol 56, 155–160.[CrossRef]
    [Google Scholar]
  11. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Lee, K. H., Bae, K. S. & Mikhailov, V. V. ( 2007; ). Arenibacter echinorum sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 57, 2655–2659.[CrossRef]
    [Google Scholar]
  12. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  13. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  14. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008573-0
Loading
/content/journal/ijsem/10.1099/ijs.0.008573-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error