1887

Abstract

An aerobic, Gram-stain-negative, rod-shaped bacterial isolate, strain NH36A, was isolated from a sandy sediment sample from the South China Sea. Colonies of the isolate were dark orange on M2 agar. Optimal growth was observed at pH 7.0–8.5, 30 °C and in the presence of 0.5–4.0 % (w/v) NaCl. The major fatty acids were C, iso-C, anteiso-C, iso-C, iso-C 3-OH, iso-C 3-OH and summed feature 3 (comprising iso-C 2-OH and/or C 7). The DNA G+C content was 38.9 mol%. 16S rRNA gene sequence analysis revealed that strain NH36A was most closely related to members of the genus , exhibiting 94.3–96.2 % sequence similarity to the type strains of species. On the basis of phenotypic, chemotaxonomic and phylogenetic data, this organism should be classified as a representative of a novel species in the genus . The name sp. nov. is proposed and the type strain is NH36A (=LMG 24842=CCTCC AB 208315=MCCC 1A04137).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008573-0
2010-01-01
2021-03-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/1/78.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008573-0&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1995; Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology . , 3rd edn. New York: John Wiley;
  2. Christakis G. B., Perlorentzou S. P., Chalkiopoulou I., Athanasiou A., Legakis N. J. 2005; Chryseobacterium indologenes non-catheter-related bacteremia in a patient with a solid tumor. J Clin Microbiol 43:2021–2023 [CrossRef]
    [Google Scholar]
  3. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  4. Ivanova E. P., Kiprianova E. A., Mikhailov V. V., Levanova G. F., Garagulya A. D., Gorshkova N. M., Vysotskii M. V., Nicolau D. V., Yumoto N. other authors 1998; Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48:247–256 [CrossRef]
    [Google Scholar]
  5. Ivanova E. P., Nedashkovskaya O. I., Chun J., Lysenko A. M., Frolova G. M., Svetashev V. I., Vysotskii M. V., Mikhailov V. V., Huq A., Colwell R. R. 2001; Arenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 51:1987–1995 [CrossRef]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  7. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  8. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003; Arenibacter troitsensis sp. nov., isolated from marine bottom sediment. Int J Syst Evol Microbiol 53:1287–1290 [CrossRef]
    [Google Scholar]
  9. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Mikhailov V. V., Bae K. S. 2004; Arenibacter certesii sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata . Int J Syst Evol Microbiol 54:1173–1176 [CrossRef]
    [Google Scholar]
  10. Nedashkovskaya O. I., Vancanneyt M., Cleenwerck I., Snauwaert C., Kim S. B., Lysenko A. M., Shevchenko L. S., Lee K. H., Park M. S. other authors 2006; Arenibacter palladensis sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata , and emended description of the genus Arenibacter . Int J Syst Evol Microbiol 56:155–160 [CrossRef]
    [Google Scholar]
  11. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Lee K. H., Bae K. S., Mikhailov V. V. 2007; Arenibacter echinorum sp. nov., isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 57:2655–2659 [CrossRef]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  13. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008573-0
Loading
/content/journal/ijsem/10.1099/ijs.0.008573-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error