sp. nov., isolated from a metal-working fluid Free

Abstract

A Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (KSS 7.8) was isolated from a water-mixed metal-working fluid. On the basis of 16S rRNA gene and sequence similarities, the isolate was clearly grouped in the genus . This allocation was confirmed by fatty acid data (major fatty acids: C 7 and C cyclo 8), polar lipid profile (major components: phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine, plus moderate amounts of phosphatidylmonomethylethanolamine and unknown aminolipid AL1), quinone system (ubiquinone Q-10) and polyamine pattern (spermidine and putrescine predominant). DNA–DNA pairing with the most closely related species showed values ranging from 24.2 to 45.7 %, and physiological and biochemical data clearly differentiated this isolate from described species. This organism represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain KSS 7.8 (=CCUG 56963=CCM 7581).

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2009-10-01
2024-03-28
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