1887

Abstract

A Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (KSS 7.8) was isolated from a water-mixed metal-working fluid. On the basis of 16S rRNA gene and sequence similarities, the isolate was clearly grouped in the genus . This allocation was confirmed by fatty acid data (major fatty acids: C 7 and C cyclo 8), polar lipid profile (major components: phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine, plus moderate amounts of phosphatidylmonomethylethanolamine and unknown aminolipid AL1), quinone system (ubiquinone Q-10) and polyamine pattern (spermidine and putrescine predominant). DNA–DNA pairing with the most closely related species showed values ranging from 24.2 to 45.7 %, and physiological and biochemical data clearly differentiated this isolate from described species. This organism represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain KSS 7.8 (=CCUG 56963=CCM 7581).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008540-0
2009-10-01
2019-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/10/2464.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008540-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Busse, H.-J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  3. Busse, H.-J., Bunka, S., Hensel, A. & Lubitz, W. ( 1997; ). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47, 698–708.[CrossRef]
    [Google Scholar]
  4. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  5. Higgins, D. G., Thompson, J. D. & Gibson, T. J. ( 1996; ). Using clustal for multiple sequence alignments. Methods Enzymol 266, 383–402.
    [Google Scholar]
  6. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  7. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  8. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J. ( 2003; ). Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P., Rosselló-Mora, R., Scholz, H., Welinder-Olsen, C. & Busse, H.-J. ( 2006; ). Description of Pseudochrobactrum gen. nov., with the two species Pseudochrobactrum asaccharolyticum sp. nov. and Pseudochrobactrum saccharolyticum sp. nov. Int J Syst Evol Microbiol 56, 1823–1829.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Scholz, H., Huber, B., Thummes, K., Busse, H.-J., Maas, E. W. & Falsen, E. ( 2007; ). Description of Pseudochrobactrum kiredjianiae sp. nov. Int J Syst Evol Microbiol 57, 755–760.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Romanenko, L. A., Tanaka, N., Frolova, G. M. & Mikhailov, V. V. ( 2008; ). Pseudochrobactrum glaciei sp. nov., a novel sea ice isolate from Peter the Great Bay of the Sea of Japan. Int J Syst Evol Microbiol 58, 2454–2458.[CrossRef]
    [Google Scholar]
  13. Scholz, H. C., Tomaso, H., Al Dahouk, S., Witte, A., Schloter, M., Kämpfer, P., Falsen, E. & Neubauer, H. ( 2006; ). Genotyping of Ochrobacterium anthropi by recA-based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis. FEMS Microbiol Lett 257, 7–16.[CrossRef]
    [Google Scholar]
  14. Stolz, A., Busse, H.-J. & Kämpfer, P. ( 2007; ). Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef]
    [Google Scholar]
  15. Tindall, B. J. ( 1990a; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  16. Tindall, B. J. ( 1990b; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef]
    [Google Scholar]
  17. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R. ( 1998; ). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008540-0
Loading
/content/journal/ijsem/10.1099/ijs.0.008540-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error