1887

Abstract

A Gram-negative, non-carbohydrate-utilizing, non-flagellated, motile, flexible, long rod-shaped, orange-pigmented bacterium, strain H6, was isolated from coastal seawater of Tianjin City, China, and its taxonomic position was investigated by using a polyphasic approach. Strain H6 grew optimally at 30 °C, in the presence of 2.0 % (w/v) NaCl and at pH 7.6. Menaquinone-6 (MK-6) was the sole respiratory quinone and the major fatty acids were iso-C (36.5 % of the total), iso-C (27.3 %) and iso-C 3-OH (10.8 %). The DNA G+C content of strain H6 was 34.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain H6 was related most closely to JCM 13821 (97.8 % similarity). DNA–DNA relatedness between the two strains was 47.4 %. Strain H6 could be further differentiated from JCM 13821 based on activity of several enzymes, hydrolysis of casein and Tweens 40, 60 and 80 and production of HS. On the basis of phenotypic, chemotaxonomic, genomic and phylogenetic data, strain H6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H6 (=CGMCC 1.7005 =JCM 15141).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008524-0
2009-10-01
2020-09-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/10/2400.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008524-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Bowman, J. P. ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surface of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa com. nov. Int J Syst Evol Microbiol 50, 1861–1868.
    [Google Scholar]
  3. Bowman, J. P., Nichols, C. M. & Gibson, J. A. ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355.[CrossRef]
    [Google Scholar]
  4. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  5. Cavalier-Smith, T. ( 2002; ). The neomuran origin of archaebacteria, the negibacterial root of the universal tree and bacterial megaclassification. Int J Syst Evol Microbiol 52, 7–76.
    [Google Scholar]
  6. Collins, M. D. ( 1994; ). Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics, pp. 265–309. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  7. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  8. Dong, X.-Z. & Cai, M.-Y. (editors) ( 2001; ). Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing: Science Press (in Chinese).
  9. Fautz, E. & Reichenbach, H. ( 1980; ). A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8, 87–91.[CrossRef]
    [Google Scholar]
  10. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  11. Grossart, H. P., Levold, F., Allgaier, M., Simon, M. & Brinkhoff, T. ( 2005; ). Marine diatom species harbour distinct bacterial communities. Environ Microbiol 7, 860–873.[CrossRef]
    [Google Scholar]
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  13. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  14. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  15. Lau, K. W., Ng, C. Y., Ren, J., Lau, S. C., Qian, P. Y., Wong, P. K., Lau, T. C. & Wu, M. ( 2005; ). Owenweeksia hongkongensis gen. nov., sp. nov., a novel marine bacterium of the phylum ‘Bacteroidetes’. Int J Syst Evol Microbiol 55, 1051–1057.[CrossRef]
    [Google Scholar]
  16. Lau, K. W., Ren, J., Wai, N. L., Qian, P.-Y., Wong, P.-K. & Wu, M. ( 2006; ). Lishizhenia caseinilytica gen. nov., sp. nov., a marine bacterium of the phylum Bacteroidetes. Int J Syst Evol Microbiol 56, 2317–2322.[CrossRef]
    [Google Scholar]
  17. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  18. O'Sullivan, L. A., Rinna, J., Humphreys, G., Weightman, A. J. & Fry, J. C. ( 2005; ). Fluviicola taffensis gen. nov., sp. nov., a novel freshwater bacterium of the family Cryomorphaceae in the phylum ‘Bacteroidetes’. Int J Syst Evol Microbiol 55, 2189–2194.[CrossRef]
    [Google Scholar]
  19. Pinhassi, J., Sala, M. M., Havskum, H., Peters, F., Guadayol, O., Malits, A. & Marrase, C. ( 2004; ). Changes in bacterioplankton composition under different phytoplankton regimens. Appl Environ Microbiol 70, 6753–6766.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Zhang, D., Yang, H., Zhang, W., Huang, Z. & Liu, S.-J. ( 2003; ). Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53, 1111–1114.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008524-0
Loading
/content/journal/ijsem/10.1099/ijs.0.008524-0
Loading

Data & Media loading...

Supplements

Scanning electron micrograph of cells of strain H6 grown on MA at 30 °C for 24 h.

IMAGE

Cellular fatty acid profiles of strain H6 and JCM 13821 . [PDF](52 KB)

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error