A Gram-positive, non-spore-forming, aerobic bacterium, designated Gsoil 001T, was isolated from the soil of a ginseng field in Pocheon province, South Korea. On the basis of 16S rRNA gene sequence analysis, strain Gsoil 001T was shown to belong to the genus Kribbella. The closest phylogenetic relatives were Kribbella koreensis LM 161T (99.0 % 16S rRNA gene sequence similarity), Kribbella flavida KACC 20248T (98.8 %) and Kribbella alba (98.8%). Lower similarity (98.4–96.9 %) was found with the other recognized species of the genus Kribbella. The major quinone was MK-9(H4), the major fatty acids were anteiso-C15 : 0 and iso-C16 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The cell-wall peptidoglycan contained ll-2,6-diaminopimelic acid. These chemotaxonomic data and the high genomic DNA G+C content (66.3 mol%) supported the affiliation of strain Gsoil 001T to the genus Kribbella. The results of physiological and biochemical tests differentiated strain Gsoil 001T genotypically and phenotypically from the Kribbella species with validly published names. Therefore, this isolate represents a novel species, for which the name Kribbella ginsengisoli sp. nov. is proposed, with the type strain Gsoil 001T (=KCTC 19134T =DSM 17941T).
CuiY.-S.,
ImW.-T.,
YinC.-R.,
LeeJ.-S.,
LeeK.-C.,
LeeS.-T.2007; Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 57:687–691[CrossRef]
EverestG. J.,
MeyersP. R.2008; Kribbella hippodromi sp. nov., isolated from soil from a racecourse in South Africa. Int J Syst Evol Microbiol 58:443–446[CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229[CrossRef]
HallT. A.1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
KirbyB. M.,
RoesM. L.,
MeyersP. R.2006; Kribbella karoonensis sp. nov. and Kribbella swartbergensis sp. nov., isolated from soil from the Western Cape, South Africa. Int J Syst Evol Microbiol 56:1097–1101[CrossRef]
LiW.-J.,
WangD.,
ZhangY.-Q.,
XuL.-H.,
JiangC.-L.2006; Kribbella yunnanensis sp. nov., Kribbella alba sp. nov., two novel species of genus Kribbella isolated from soils in Yunnan, China. Syst Appl Microbiol 29:29–35[CrossRef]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
MinnikinD. E.,
PatelP. V.,
AlshamaonyL.,
GoodfellowM.1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117[CrossRef]
MooreD. D.,
DowhanD.1995; Preparation and analysis of DNA. In Current Protocols in Molecular Biology pp 2–11 Edited by
AusubelF. W.,
BrentR.,
KingstonR. E.,
MooreD. D.,
SeidmanJ. G.,
SmithJ. A.,
StruhlK.
New York: Wiley;
SohnK.,
HongS.-G.,
BaeK.-S.,
ChunJ.2003; Transfer of Hongia koreensis Lee et al. 2000 to the genus Kribbella Park et al. 1999 as Kribbella koreensis comb. nov. Int J Syst Evol Microbiol 53:1005–1007[CrossRef]
TenL. N.,
ImW.-T.,
KimM.-K.,
KangM.-S.,
LeeS.-T.2004; Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J Microbiol Methods 56:375–382[CrossRef]
TenL. N.,
JungH.-M.,
YooS.-A.,
ImW.-T.,
LeeS.-T.2008; Lysobacter daecheongensis sp. nov., isolated from sediment of stream near the Daechung dam in South Korea. J Microbiol 46:519–524[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
TrujilloM. E.,
KroppenstedtR. M.,
SchumannP.,
Martínez-MolinaE.2006; Kribbella lupini sp. nov., isolated from the roots of Lupinus angustifolius
. Int J Syst Evol Microbiol 56:407–411[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]