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Abstract

A Gram-positive, non-spore-forming, aerobic bacterium, designated Gsoil 001, was isolated from the soil of a ginseng field in Pocheon province, South Korea. On the basis of 16S rRNA gene sequence analysis, strain Gsoil 001 was shown to belong to the genus . The closest phylogenetic relatives were LM 161 (99.0 % 16S rRNA gene sequence similarity), KACC 20248 (98.8 %) and (98.8%). Lower similarity (98.4–96.9 %) was found with the other recognized species of the genus . The major quinone was MK-9(H), the major fatty acids were anteiso-C and iso-C and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The cell-wall peptidoglycan contained -2,6-diaminopimelic acid. These chemotaxonomic data and the high genomic DNA G+C content (66.3 mol%) supported the affiliation of strain Gsoil 001 to the genus . The results of physiological and biochemical tests differentiated strain Gsoil 001 genotypically and phenotypically from the species with validly published names. Therefore, this isolate represents a novel species, for which the name sp. nov. is proposed, with the type strain Gsoil 001 (=KCTC 19134 =DSM 17941).

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2010-02-01
2019-12-12
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Supplements

vol. , part 2, pp. 364 - 368

Comparison of selected characteristics of strain Gsoil 001 with those of its closest relative, KACC 20250 .

Results from five replications of DNA–DNA hybridization between strain Gsoil 001 and the type strains of species.

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