A novel aerobic, non-motile, Gram-positive, pink–orange-pigmented, coccoid marine bacterium, designated strain SJ2, was isolated from the Gwangalli coast of Korea. The taxonomic position of strain SJ2 was determined based on 16S rRNA gene sequence analysis, fatty acid patterns and physiological reaction profiles. The full-length 16S rRNA gene sequence of strain SJ2 showed highest similarity to those of the type strains of and . Strain SJ2 exhibited mean levels of DNA–DNA relatedness of 17 and 35 % to the type strains of and , respectively. Based on these results, strain SJ2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SJ2 (=KCCM 42914 =LMG 24672).


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  1. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  4. Hugh, R. & Leifson, E.(1953). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66, 24–26. [Google Scholar]
  5. Kim, S. B., Nedashkovskaya, O. I., Mikhailov, V. V., Han, S. K., Kim, K.-O., Rhee, M. S. & Bae, K. S.(2004).Kocuria marina sp. nov., a novel actinobacterium isolated from marine sediment. Int J Syst Evol Microbiol 54, 1617–1620.[CrossRef] [Google Scholar]
  6. Kovács, G., Burghardt, J., Pradella, S., Schumann, P., Stackebrandt, E. & Màrialigeti, K.(1999).Kocuria palustris sp. nov. and Kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (Typha angustifolia). Int J Syst Bacteriol 49, 167–173.[CrossRef] [Google Scholar]
  7. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  8. Li, W.-J., Chen, H.-H., Xu, P., Zhang, Y.-O., Schumann, P., Tang, S.-K., Xu, L.-H. & Jiang, C.-L.(2004).Yania halotolerans gen. nov., sp. nov., a novel member of the suborder Micrococcineae from saline soil in China. Int J Syst Evol Microbiol 54, 525–531.[CrossRef] [Google Scholar]
  9. Li, W.-J., Zhang, Y.-Q., Schumann, P., Chen, H.-H., Hozzein, W. N., Tian, X.-P., Xu, L.-H. & Jiang, C.-L.(2006).Kocuria aegyptia sp. nov., a novel actinobacterium isolated from a saline, alkaline desert soil in Egypt. Int J Syst Evol Microbiol 56, 733–737.[CrossRef] [Google Scholar]
  10. Lipski, A. & Altendorf, K.(1997). Identification of heterotrophic bacteria isolated from ammonia-supplied experimental biofilters. Syst Appl Microbiol 20, 448–457.[CrossRef] [Google Scholar]
  11. Mayilraj, S., Kroppenstedt, R. M., Suresh, K. & Saini, H. S.(2006).Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56, 1971–1975.[CrossRef] [Google Scholar]
  12. Rainey, F. A., Dorsch, M., Morgan, H. W. & Stackebrandt, E.(1992). 16S rDNA analysis of Spirochaeta thermophila: its phylogenetic position and implications for the systematics of the order Spirochaetales. Syst Appl Microbiol 15, 197–202. [Google Scholar]
  13. Reddy, G. S. N., Prakash, J. S. S., Prabahar, V., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S.(2003).Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 53, 183–187.[CrossRef] [Google Scholar]
  14. Sasser, M.(1990). Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology, pp. 199–204. Edited by Z. Klement, K. Rudolph & D. C. Sands. Budapest: Akademiai Kiado.
  15. Skerman, V. B. D.(1967).A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  16. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  17. Stackebrandt, E., Koch, C., Gvozdiak, O. & Schumann, P.(1995). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45, 628–692. [Google Scholar]
  18. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  19. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  20. Tvrzová, L., Schumann, P., Sedláček, I., Páčová, Z., Spröer, C., Verbarg, S. & Kroppenstedt, R. M.(2005). Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. nov. Int J Syst Evol Microbiol 55, 139–142.[CrossRef] [Google Scholar]
  21. Zhou, G., Luo, X., Tang, Y., Zhang, L., Yang, Q., Qiu, Y. & Fang, C.(2008).Kocuria flava sp. nov. and Kocuria turfanensis sp. nov., airborne actinobacteria isolated from Xinjiang, China. Int J Syst Evol Microbiol 58, 1304–1307.[CrossRef] [Google Scholar]

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Transmission electron micrograph of cells of sp. nov. SJ2 during the exponential stage of growth. Strain SJ2 was cultivated for 2 days at 25 °C on modified trypticase soy agar (2 % NaCl added) adjusted to pH 8.0. Bar, 200 nm.


Maximum-parsimony tree based on 16S rRNA gene sequence analysis of members of the genus . [PDF](48 KB)


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