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Volume 59, Issue 9

sp. nov., isolated from a marine solar saltern, and emended description of the genus Free

Abstract

A Gram-negative, motile and rod-shaped bacterial strain, designated ISL-43, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-43 grew optimally at pH 7.0–8.0 and 30 °C and in the presence of approximately 2 % NaCl. It contained Q-8 as the predominant ubiquinone and C, C 8, C 7 and/or iso-C 2-OH, and C 7 as the major fatty acids. The DNA G+C content was 58.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ISL-43 fell within the genus , clustering with M18 with a bootstrap value of 100 %. Strain ISL-43 exhibited 16S rRNA gene sequence similarity values of 98.1 and 95.5 % to M18 and M9, respectively. Strain ISL-43 exhibited DNA–DNA relatedness values of 17 and 10 % to KCTC 12357 and KCTC 12356, respectively. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-43 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is ISL-43 (=KCTC 22484=CCUG 56757).

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2009-09-01
2024-04-24
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References

  1. Baumann, P. & Baumann, L.(1981). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer-Verlag.
  2. Bruns, A., Rohde, M. & Berthe-Corti, L.(2001).Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef] [Google Scholar]
  3. Cohen-Bazire, G., Sistrom, W. R. & Stanier, R. Y.(1957). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49, 25–68.[CrossRef] [Google Scholar]
  4. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  6. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  7. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  8. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  9. Lim, J.-M., Jeon, C. O., Lee, J.-C., Song, S.-M., Kim, K.-Y. & Kim, C.-J.(2006).Marinimicrobium koreense gen. nov., sp. nov. and Marinimicrobium agarilyticum sp. nov., novel moderately halotolerant bacteria isolated from tidal flat sediment in Korea. Int J Syst Evol Microbiol 56, 653–657.[CrossRef] [Google Scholar]
  10. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note no. 1. Newark, DE: MIDI.
  11. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  12. Staley, J. T.(1968).Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95, 1921–1942. [Google Scholar]
  13. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  14. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  15. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef] [Google Scholar]
  16. Yoon, J.-H., Lee, S. T. & Park, Y.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef] [Google Scholar]
  17. Yoon, J.-H., Kang, K. H. & Park, Y.-H.(2003).Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef] [Google Scholar]
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Marinimicrobium locisalis sp. nov., isolated from a marine solar saltern, and emended description of the genus Marinimicrobium
Int J Syst Evol Microbiol 59, 2260 (2009); https://doi.org/10.1099/ijs.0.008458-0
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