1887

Abstract

A facultatively anaerobic, nitrogen-fixing bacterium, strain MSSRF40, was isolated from roots of mangrove-associated wild rice ( Tateoka). On the basis of 16S rRNA gene sequence similarities, strain MSSRF40 was shown to belong to the family , most closely related to E603 (97.2 % sequence similarity), subsp. LMG 2683 (97.1 %), . D5/23 (97.1 %) and . EN-119 (97.0 %). Sequence analysis of , and genes showed that strain MSSRF40 had relatively low sequence similarity (<91, <84 and <90 %) to recognized species of different genera of the family and formed an independent phyletic lineage in all phylogenetic analyses using the 16S rRNA, , and genes, clearly indicating that strain MSSRF40 could not be affiliated to any of the recognized genera within the family . The dominant cellular fatty acids were C, C 7 and/or iso-C 2-OH and C 7, similar to those of other members of the . The DNA G+C content was 50.1 mol%. Phylogenetic distinctiveness and phenotypic differences from its phylogenetic neighbours indicated that strain MSSRF40 represents a novel species and genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is strain MSSRF40 (=LMG 24236 =DSM 19579).

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2010-01-01
2019-10-20
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Amplification and sequencing primers used in this study. [PDF](75 KB)

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Maximum-parsimony tree constructed using 16S rRNA gene sequences of gen. nov., sp. nov. MSSRF40 and related members of the . Bootstrap values (percentages of 1000 replicates) >50% are shown. The tree is presented as supplied by the author.

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Neighbour-joining trees comprising (Fig. S2), (Fig. S3) and (Fig. S4) gene sequences of gen. nov., sp. nov. MSSRF40 and related members of the . Bootstrap values (percentages of 1000 replicates) >60% are shown. Bars, 0.01% (Fig. S2), 0.02% (Fig. S3) and 0.05% (Fig. S4) nucleotide substitutions. The trees are presented as supplied by the author.

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Electron micrograph of negatively stained cell of strain MSSRF40 harvested after 24 h from Luria–Bertani broth. Individual bacteria exhibit peritrichous flagella. Original magnification ×25 000.

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