A strain, IMCC1736, was isolated recently from a Korean freshwater pond following an extensive study of the microbial community in this ecosystem. Its 16S rRNA gene was sequenced and phylogenetic analysis placed this strain within the group, closely related to NRIC 0604, with 97.9 % sequence similarity. In the present work, the taxonomic status of strain IMCC1736 has been re-evaluated. It was characterized phylogenetically, genotypically and phenotypically and, based on DNA–DNA hybridization values, this strain represents a novel species. Strain IMCC1736 can be differentiated genotypically from its closest relatives by randomly amplified polymorphic DNA analysis and ribotyping patterns; phenotypically, it can be distinguished by its inability to grow in 5 % NaCl and at pH 3.3 and by certain carbohydrate fermentations. Strain IMCC1736 is Gram-positive, catalase-negative and microaerophilic. Cells are motile rods and show homofermentative metabolism. The name sp. nov. is proposed, with strain IMCC1736 (=CECT 7355 =DSM 21051) as the type strain.


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vol. , part 9, pp. 2215 - 2218

Phenotypic characteristics of strain IMCC1736 ( sp nov.) and closely related species. [PDF](60 KB)

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