1887

Abstract

A Gram-negative, rod-shaped, purple non-sulfur bacterial strain, designated JA415, was isolated from the mud of a pokkali rice field located on Vypeen Island, Ernakulam, Kerala, India. Strain JA415 was motile by means of a single polar flagellum. Photo- and chemo-organoheterotrophic growth was observed using organic compounds as carbon sources and electron donors. Photo- and chemolithoautotrophic growth using thiosulfate as electron donor did not occur. Fermentative growth could not be demonstrated. Intracellular photosynthetic membranes were lamellar stacks parallel to the cytoplasmic membrane. Bacteriochlorophyll and carotenoids of the spirilloxanthin series were present as photosynthetic pigments. Niacin, pantothenate and -aminobenzoate were required as growth factors. C 7 was the predominant cellular fatty acid component. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JA415 clustered with members of the genus in the class . On the basis of phenotypic and molecular genetic evidence, it is proposed that strain JA415 be classified as a representative of a novel species of the genus , family , with the name sp. nov. The type strain is JA415 (=KCTC 5711 =NBRC 104972).

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2009-09-01
2019-10-22
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Phase-contrast micrograph of cells of strain JA415 . Bar, 5 µm.

IMAGE

Electron micrographs of a negatively stained cell of strain JA145 showing the single polar flagellum (a) and ultrathin sections of cells of strain JA415 showing the lamellar nature of the photosynthetic membranes extending throughout the cell (b). Bars, 1.3 µm (a), 167 nm (b, left) and 125 nm (b, right).

IMAGE

Electron micrographs of a negatively stained cell of strain JA145 showing the single polar flagellum (a) and ultrathin sections of cells of strain JA415 showing the lamellar nature of the photosynthetic membranes extending throughout the cell (b). Bars, 1.3 µm (a), 167 nm (b, left) and 125 nm (b, right).

IMAGE

Whole-cell absorption spectrum of strain JA415 .

IMAGE

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