gen. nov., sp. nov. and sp. nov., halophilic, alkalithermophilic micro-organisms from soda lakes of the Wadi An Natrun, Egypt Free

Abstract

Novel strains of anaerobic, halophilic, alkalithermophilic bacteria were isolated from sediments of the alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Strains JW/NM-WN-LU (and related strains) and JW/NM-WN-LH1 were non-spore-forming, non-motile bacteria with a Gram-type positive cell wall. Optimal growth of strain JW/NM-WN-LU occurred at pH 9.5, 52 °C and with 3.7 M Na (2.2 M added NaCl). Optimal growth of strain JW/NM-WN-LH1 occurred at pH 9.9, 51 °C and with 3.9 M Na (2.3 M added NaCl). Both strains were obligately anaerobic and chemoorganotrophic; producing lactate and acetate as organic acids from pyruvate in different ratios. The DNA G+C contents of strains JW/NM-WN-LU and JW/NM-WN-LH1 were 41.0 and 42.0 mol%, respectively. The main cellular fatty acids in both strains were anteiso-branched 15 : 0 and iso-branched 15 : 0. Phylogenetic analysis showed that both novel isolates belonged to the family within the order . Based on genotypic and phenotypic data, strain JW/NM-WN-LU (=DSM 18760=ATCC BAA-1443) represents the type strain of a novel species, sp. nov. Strain JW/NM-WN-LH1 represents a novel genus and species within the family , gen. nov., sp. nov. The type strain of the type species is JW/NM-WN-LH1 (=DSM 18770=ATCC BAA-1444).

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2009-08-01
2024-03-29
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