1887

Abstract

A Gram-negative-staining bacterium, designated strain 25B, was isolated from a soil sample from a rice field in Guangxi Province, China, and its taxonomic position was investigated by using a polyphasic approach. Cells were rod-shaped, non-spore-forming, non-motile and strictly aerobic. Strain 25B grew optimally at 37 °C and pH 7.0. The predominant fatty acids of this soil isolate were C 7, C cyclo 8 and C. Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence showed that strain 25B formed a monophyletic clade with the type strain of ; the two organisms shared 97.2 % 16S rRNA gene sequence similarity. However, the two strains shared low DNA–DNA relatedness. Strain 25B was also readily distinguishable from DSM 14364 by various phenotypic characteristics. The combination of genotypic and phenotypic data suggests that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 25B (=CGMCC 1.7666=JCM 15710).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007997-0
2009-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/8/1997.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007997-0&mimeType=html&fmt=ahah

References

  1. Andrews, K. T. & Patel, B. K. C.(1996).Fervidobacterium gondwanense sp. nov., a new thermophilic anaerobic bacterium isolated from nonvolcanically heated geothermal waters of the Great Artesian Basin of Australia. Int J Syst Bacteriol 46, 265–269.[CrossRef] [Google Scholar]
  2. Auling, G., Busse, H. J., Egli, T., El-Banna, T. & Stackebrandt, E.(1993). Description of the Gram-negative, obligately aerobic, nitrilotriacetate (NTA)-utilizing bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asaccharovorans, gen. nov., sp. nov. Syst Appl Microbiol 16, 104–112. [Google Scholar]
  3. Das, S. K., Mishra, A. K., Tindall, B. J., Rainey, F. A. & Stackebrandt, E.(1996). Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (strain BI-42) gen. nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing. Int J Syst Bacteriol 46, 981–987.[CrossRef] [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurements of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  5. Egli, T., Weilenmann, H.-U., El-Banna, T. & Auling, G.(1988). Gram-negative, aerobic, nitrilotriacetate-utilizing bacteria from wastewater and soil. Syst Appl Microbiol 10, 297–305.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  7. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(1993).phylip (phylogeny inference package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef] [Google Scholar]
  10. Garrity, G. M. & Holt, J. G.(2001). The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 119–166. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  11. Garrity, G. M., Bell, J. A. & Lilburn, T.(2005a). Class I. Alphaproteobacteria class. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, The Proteobacteria, part C, The Alpha-, Beta-, Delta-, and Epsilonproteobacteria, p. 1. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  12. Garrity, G. M., Bell, J. A. & Lilburn, T.(2005b). Family IX. Methylobacteriaceae fam. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, The Proteobacteria, part C, The Alpha-, Beta-, Delta-, and Epsilonproteobacteria, p. 567. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  13. Goodfellow, M. & Orchard, V. A.(1974). Antibiotic sensitivity of some nocardioform bacteria and its value as a criterion for taxonomy. J Gen Microbiol 83, 375–387.[CrossRef] [Google Scholar]
  14. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  15. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  16. Jahnke, K. D.(1992).basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef] [Google Scholar]
  17. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  18. Kanso, S. & Patel, B. K. C.(2003).Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer. Int J Syst Evol Microbiol 53, 401–406.[CrossRef] [Google Scholar]
  19. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  20. Kuykendall, L. D.(2005). Order VI. Rhizobiales ord. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, The Proteobacteria, part C, The Alpha-, Beta-, Delta-, and Epsilonproteobacteria, p. 324. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  21. La Scola, B., Mallet, M. N., Grimont, P. A. D. & Raoult, D.(2003).Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996). Int J Syst Evol Microbiol 53, 15–20.[CrossRef] [Google Scholar]
  22. Lu, Z., Liu, Z., Wang, L., Zhang, Y., Qi, W. & Goodfellow, M.(2001).Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp. nov., novel actinomycetes from soil. Int J Syst Evol Microbiol 51, 319–325. [Google Scholar]
  23. Marmur, J. & Doty, P.(1962). Determination of base composition of deoxyribonucleic acid from its denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  24. Mulder, E. G. & Deinema, M. H.(1992). The sheathed bacteria. In The Prokaryotes, 2nd edn, pp. 2612–2624. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  25. Patt, T. E., Cole, G. C. & Hanson, R. S.(1976).Methylobacterium, a new genus of facultatively methylotrophic bacteria. Int J Syst Bacteriol 26, 226–229.[CrossRef] [Google Scholar]
  26. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  27. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  28. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  29. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  30. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  31. Urakami, T., Araki, H., Suzuki, K. & Komagata, K.(1993). Further studies of the genus Methylobacterium and description of Methylobacterium aminovorans sp. nov. Int J Syst Bacteriol 43, 504–513.[CrossRef] [Google Scholar]
  32. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  33. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J.(1989). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178. [Google Scholar]
  34. Wynter, C., Patel, B. K., Bain, P., de Jersey, J., Hamilton, S. & Inkerman, P. A.(1996). A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the Great Artesian Basin of Australia. FEMS Microbiol Lett 140, 271–276.[CrossRef] [Google Scholar]
  35. Zhang, J., Zhang, Y., Xiao, C., Liu, Z. & Goodfellow, M.(2002).Rhodococcus maanshanensis sp. nov., a novel actinomycete from soil. Int J Syst Evol Microbiol 52, 2121–2126.[CrossRef] [Google Scholar]
  36. Zhang, J., Wu, D., Zhang, J., Liu, Z. & Song, F.(2008).Saccharopolyspora shandongensis sp. nov., isolated from wheat-field soil. Int J Syst Evol Microbiol 58, 1094–1099.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007997-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007997-0
Loading

Data & Media loading...

Supplements

vol. , part 8, pp. 1997 - 2001

Fatty acid contents of strain 25B ( sp. nov.) and the type strain of . [PDF](21 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error