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A Gram-negative-staining bacterium, designated strain 25BT, was isolated from a soil sample from a rice field in Guangxi Province, China, and its taxonomic position was investigated by using a polyphasic approach. Cells were rod-shaped, non-spore-forming, non-motile and strictly aerobic. Strain 25BT grew optimally at 37 °C and pH 7.0. The predominant fatty acids of this soil isolate were C18 : 1 ω7c, C19 : 0 cyclo ω8c and C16 : 0. Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence showed that strain 25BT formed a monophyletic clade with the type strain of Microvirga subterranea; the two organisms shared 97.2 % 16S rRNA gene sequence similarity. However, the two strains shared low DNA–DNA relatedness. Strain 25BT was also readily distinguishable from Microvirga subterranea DSM 14364T by various phenotypic characteristics. The combination of genotypic and phenotypic data suggests that the isolate represents a novel species of the genus Microvirga, for which the name Microvirga guangxiensis sp. nov. is proposed. The type strain is 25BT (=CGMCC 1.7666T=JCM 15710T).
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International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 8, pp. 1997 - 2001
Supplementary Table S1. Fatty acid contents of strain 25B T( M. guangxiensis sp. nov.) and the type strain of Microvirga subterranea . [PDF](21 KB)