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Abstract

An orange-pigmented, non-motile, coccoid bacterial strain, designated TR0125, was isolated from dust samples collected in the high atmosphere above Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was within the radiation of species. The major peptidoglycan amino acids were -glutamic acid, glycine, -alanine, -alanine and ornithine. The predominant fatty acids were iso-C, iso-C 9 and iso-C. Strong resistance to desiccation, UV-C and gamma radiation and high DNA G+C content also supported the affiliation of strain TR0125 to the genus . Strain TR0125 showed the highest 16S rRNA gene sequence similarity value (95.7 %) to the type strain of , and phylogenetic analysis showed that it was further separated from than from , indicating that strain TR0125 was not a member of these two species. In addition, phenotypic differences were found between strain TR0125 and the type strains of these two species. Therefore, a novel species of the genus , sp. nov. (type strain, TR0125=JCM 11750=DSM 21212), is proposed to accommodate this isolate.

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2009-08-01
2019-12-07
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References

  1. Arrage, A. A., Phelps, T. J., Benoit, R. E., Palumbo, A. V. & White, D. C. ( 1993; ). Bacterial sensitivity to UV light as a model for ionizing radiation resistance. J Microbiol Methods 18, 127–136.[CrossRef]
    [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Brooks, B. W. & Murray, R. G. E. ( 1981; ). Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int J Syst Bacteriol 31, 353–360.[CrossRef]
    [Google Scholar]
  4. Callegan, R. P., Nobre, M. F., McTernan, P. M., Battista, J. R., Navarro-Gonzalez, R., McKay, C. P., da Costa, M. S. & Rainey, F. A. ( 2008; ). Description of four novel psychrophilic, ionizing radiation-sensitive Deinococcus species from alpine environments. Int J Syst Evol Microbiol 58, 1252–1258.[CrossRef]
    [Google Scholar]
  5. Christensen, E. A. & Kristensen, H. ( 1981; ). Radiation-resistance of micro-organisms from air in clean premises. Acta Pathol Microbiol Scand [B] 89, 293–301.
    [Google Scholar]
  6. Jobb, G., von Haeseler, A. & Strimmer, K. ( 2004; ). TREEFINDER: a powerful graphical analysis environment for molecular phylogenetics. BMC Evol Biol 4, 18 [CrossRef]
    [Google Scholar]
  7. Komagata, K. & Suzuki, K. I. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. In Methods in Microbiology, pp. 161–207. Edited by R. R. Colwell & R. Grigorova. London: Academic Press.
  8. Kuykendall, L. D., Roy, M. A., O'Neill, J. J. & Devine, T. E. ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef]
    [Google Scholar]
  9. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  10. Miller, J. H. ( 1992; ). A Short Course in Bacterial Genetics: a Laboratory Manual and Handbook for Escherichia coli. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  11. Murray, R. G. E. & Robinow, C. F. ( 1958; ). Cytological studies of a tetrad-forming coccus, 7th edn. Stockholm: Proc Intern Congr Microbiol pp. 427–428.
  12. Rainey, F. A., Nobre, M. F., Schumann, P., Stackebrandt, E. & da Costa, M. S. ( 1997; ). Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison. Int J Syst Bacteriol 47, 510–514.[CrossRef]
    [Google Scholar]
  13. Rainey, F. A., Ray, K., Ferreira, M., Gatz, B. Z., Nobre, M. F., Bagaley, D., Rash, B. A., Park, M. J., Earl, A. M. & other authors ( 2005; ). Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. Appl Environ Microbiol 71, 5225–5235.[CrossRef]
    [Google Scholar]
  14. Satoh, K., Ohba, H., Sghaier, H. & Narumi, I. ( 2006; ). Down-regulation of radioresistance by LexA2 in Deinococcus radiodurans. Microbiology 152, 3217–3226.[CrossRef]
    [Google Scholar]
  15. Shimada, H., Shida, Y., Nemoto, N., Oshima, T. & Yamagishi, A. ( 2001; ). Quinone profiles of Thermoplasma acidophilum HO-62. J Bacteriol 183, 1462–1465.[CrossRef]
    [Google Scholar]
  16. Takami, H., Kobata, K., Nagahama, T., Kobayashi, H., Inoue, A. & Horikoshi, K. ( 1999; ). Biodiversity in deep-sea sites located near the south part of Japan. Extremophiles 3, 97–102.[CrossRef]
    [Google Scholar]
  17. Tatusova, T. A. & Madden, T. L. ( 1999; ). blast 2 sequences, a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174, 247–250.[CrossRef]
    [Google Scholar]
  18. Tindall, B. J. ( 1989; ). Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum. FEMS Microbiol Lett 60, 251–254.[CrossRef]
    [Google Scholar]
  19. Weon, H. Y., Kim, B. Y., Schumann, P., Son, J. A., Jang, J., Go, S. J. & Kwon, S. W. ( 2007; ). Deinococcus cellulosilyticus sp. nov., isolated from air. Int J Syst Evol Microbiol 57, 1685–1688.[CrossRef]
    [Google Scholar]
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Differential characteristics of strain TR0125 ( sp. nov.) and type strains of the most closely related species. [PDF](18 KB)

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Fatty acid compositions of strain TR0125 ( sp. nov.) and type strains of the most closely related species. [PDF](45 KB)

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