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Volume 59, Issue 7

sp. nov., isolated from a freshwater lake Free

Abstract

A yellow-pigmented, rod-shaped, Gram-staining-negative, aerobic bacterial strain, designated CJ7, was isolated from a freshwater lake at Chung-Ang University in Anseong, South Korea. Results from 16S rRNA gene sequence analysis revealed that the isolate was phylogenetically affiliated with members of the genus . Strain CJ7 showed sequence similarity values of 91.5–98.0 % to the type strains of recognized species. The DNA–DNA relatedness between strain CJ7 and DSM 18292, which showed the highest 16S rRNA gene sequence similarity, was 25.4 %. The predominant cellular fatty acids were iso-C (18.3 %), summed feature 3 (comprising iso-C 2-OH and/or C 7) (16.1 %), iso-C 3-OH (8.9 %), iso-C 3-OH (7.2 %), iso-C 9 (6.1 %) and iso-C (5.9 %). Flexirubin-type pigments were absent. The major isoprenoid quinone was menaquinone-6. The DNA G+C content was 34.5 mol%. The results obtained from this study, with a polyphasic taxonomic approach, suggested that strain CJ7 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is CJ7 (=KACC 13353=JCM 15651).

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2009-07-01
2023-12-08
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References

  1. Anacker, R. L. & Ordal, E. J.(1955). Study of a bacteriophage infecting the myxobacterium Chondrococcus columnaris. J Bacteriol 70, 738–741. [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A.(1993).Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Bernardet, J. F. & Bowman, J. P.(2006). The genus Flavobacterium. In The Prokaryotes. A Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 481–531. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer-Verlag.
  4. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  5. Bernardet, J. F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  6. Chun, J., Lee, J. H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequence. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  7. Collins, M. D.(1985). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366. [Google Scholar]
  8. Cousin, S., Päuker, O. & Stackebrandt, E.(2007).Flavobacterium aquidurense sp. nov. and Flavobacterium hercynium sp. nov., from a hard-water creek. Int J Syst Evol Microbiol 57, 243–249.[CrossRef] [Google Scholar]
  9. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  11. Felsenstein, J.(2005).phylip (phylogeny inference package) version 3.6. Distributed by the author. University of Washington, Seattle, USA.
  12. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  13. Hsu, S. C. & Lockwood, J. L.(1975). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29, 422–426. [Google Scholar]
  14. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  15. Jeon, Y.-S., Chung, H., Park, S., Hur, I., Lee, J.-H. & Chun, J.(2005). jPHYDIT: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21, 3171–3173.[CrossRef] [Google Scholar]
  16. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  17. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  18. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  19. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athayle, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  20. Nogi, Y., Soda, K. & Oikawa, T.(2005).Flavobacterium frigidimaris sp. nov., isolated from Antarctic seawater. Syst Appl Microbiol 28, 310–315.[CrossRef] [Google Scholar]
  21. Paton, A. M.(1959). An improved method for preparing pectate gels. Nature 183, 1812–1813. [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Washington, DC: American Society for Microbiology.
  24. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today,152–155. [Google Scholar]
  25. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  26. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  27. West, P. A. & Colwell, R. R.(1984). Identification and classification of the Vibrionaceae – an overview. In Vibrios in the Environment, pp. 285–363. Edited by R. R. Colwell. New York: Wiley.
  28. Yi, H. & Chun, J.(2006).Thalassobius aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 44, 171–176. [Google Scholar]
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Flavobacterium chungangense sp. nov., isolated from a freshwater lake
Int J Syst Evol Microbiol 59, 1754 (2009); https://doi.org/10.1099/ijs.0.007955-0
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vol. , part 7, pp. 1754 - 1758

Transmission electron micrograph of a negatively stained cell of strain CJ7 grown on R2A at 30°C for 2 days. Bar, 0.5µm.

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