1887

Abstract

Two aerobic, non-motile and non-sporulating bacteria, strains LY1 and L462, were recovered from a hot spring located in the Qianshan area of north-eastern China. The novel strains had Gram-negative cell walls and grew at 30–66 °C (optimum 55–60 °C) and pH 5.5–10.0 (optimum pH 8.0–8.5).16S rRNA gene sequence similarity analysis revealed that strains LY1 and L462 were most closely related to ATCC 35948, WR-30 and GY-1, with 97.1–98.4 % sequence similarity. Phylogenetic analysis indicated that the new isolates represented a novel species by forming a distinctive lineage within genus , which was confirmed by DNA–DNA reassociation values. Strain LY1 was obviously different from its closest relatives in a number of phenotypic characteristics, such as the inability to hydrolyse casein or to assimilate melibiose, trehalose, sucrose and lactose. Strain L462 was distinct in its ability to reduce nitrate. In addition, the novel strains were distinct in having larger amounts of anteiso-C than their closest phylogenetic neighbours. On the basis of this polyphasic taxonomic characterization, the name sp. nov. is proposed for the novel species, currently represented by the type strain LY1 (=CGMCC 1.6990 =JCM 15151) and strain L462 (=CGMCC 1.6989 =JCM 15150).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007914-0
2010-04-01
2020-09-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/4/840.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007914-0&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805
    [Google Scholar]
  2. Chen C. Y., Lin L. B., Peng Q., Ben K. L., Zhou Z. M. 2002a; Meiothermus rosaceus sp. nov. isolated from Tengchong hot spring in Yunnan, China. FEMS Microbiol Lett 216:263–268
    [Google Scholar]
  3. Chen M. Y., Lin G. H., Lin Y. T., Tsay S. S. 2002b; Meiothermus taiwanensis sp. nov., a novel filamentous, thermophilic species isolated in Taiwan. Int J Syst Evol Microbiol 52:1647–1654
    [Google Scholar]
  4. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  5. Chung A. P., Rainey F., Nobre M. F., Burghardt J., da Costa M. S. 1997; Meiothermus cerbereus sp. nov., a new slightly thermophilic species with high levels of 3-hydroxy fatty acids. Int J Syst Bacteriol 47:1225–1230 [CrossRef]
    [Google Scholar]
  6. da Costa M. S., Rainey F. A., Nobre M. F. 2006; The genus Thermus and relatives. In The Prokaryotes: a Handbook on the Biology of Bacteria , 3rd edn. vol 7 pp 797–812 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1993 phylip (phylogeny inference package) version 3.5.1 Distributed by the author Department of Genome Sciences,; University of Washington; Seattle, USA:
    [Google Scholar]
  9. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  10. Hudson J. A., Morgan H. W., Daniel R. M. 1986; A numerical classification of some Thermus isolates. J Gen Microbiol 132:531–540
    [Google Scholar]
  11. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  12. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  13. Loginova L. G., Egorova L. A., Golovacheva R. S., Seregina L. M. 1984; Thermus ruber sp. nov., nom. rev. Int J Syst Bacteriol 34:498–499 [CrossRef]
    [Google Scholar]
  14. Manaia C. M., da Costa M. S. 1991; Characterization of halotolerant Thermus isolates from shallow marine hot springs on S. Miquel, Azores. J Gen Microbiol 137:2643–2648 [CrossRef]
    [Google Scholar]
  15. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  16. Nobre M. F., da Costa M. S. 2001; Genus II. Meiothermus . In Bergey s Manual of Systematic Bacteriology , 2nd edn. vol 1 pp 414–420 Edited by Boone D. R., Castenholtz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  17. Nobre M. F., Trüper H. G., da Costa M. S. et al. 1996; Transfer of Thermus ruber (Loginova et al. 1984), Thermus silvanus (Tenreiro et al. 1995), and Thermus chliarophilus (Tenreiro et al. 1995) to Meiothermus gen. nov. as Meiothermus ruber comb. nov., Meiothermus silvanus comb. nov., and Meiothermus chliarophilus comb. nov., respectively, and emendation of the genus Thermus . Int J Syst Bacteriol 46:604–606 [CrossRef]
    [Google Scholar]
  18. Pires A. L., Albuquerque L., Tiago I., Nobre M. F., Empadinhas N., Veríssimo A., da Costa M. S. 2005; Meiothermus timidus sp. nov., a new slightly thermophilic yellow-pigmented species. FEMS Microbiol Lett 245:39–45 [CrossRef]
    [Google Scholar]
  19. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  22. Tenreiro S., Nobre M. F., da Costa M. S. 1995; Thermus silvanus sp. nov. and Thermus chliarophilus sp. nov., two new species related to Thermus ruber but with lower growth temperatures. Int J Syst Bacteriol 45:633–639 [CrossRef]
    [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  24. Tindall B. J. 1989; Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum . FEMS Microbiol Lett 60:251–254 [CrossRef]
    [Google Scholar]
  25. Tindall B. J. 1991; Lipid composition of Rhodothermus marinus . FEMS Microbiol Lett 80:65–68 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007914-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007914-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error