1887

Abstract

A novel cold-living, Gram-stain-negative, yellow-pigmented and obligately aerobic bacterium designated strain 0563 was isolated from the China No. 1 glacier. Phylogenetic analysis showed that strain 0563 was a member of the genus , sharing the highest 16S rRNA gene sequence similarities with JCM 11473 (98.5 %) and NBRC 102679 (98.2 %). The genomic DNA G+C content was 34.8 mol%. Strain 0563 displayed the common phenotypic and chemotaxonomic features of the genus , containing menaquinone-6 (MK-6) as the major quinone and iso-C, iso-C 3-OH, summed feature 3 (comprising C 7 and/or C 6), C 6, iso-C G and iso-C 3-OH as the major fatty acids. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, the novel species p. nov. is proposed; the type strain is 0563 (=CGMCC 1.6847 =JCM 15087).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007906-0
2009-11-01
2019-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2773.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007906-0&mimeType=html&fmt=ahah

References

  1. Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W. & Huntoon, F. M. ( 1923; ). Genus II. Flavobacterium gen. nov. In Bergey's Manual of Determinative Bacteriology, pp. 97–117. Baltimore: Williams & Wilkins.
  2. Bernardet, J. F. & Bowman, J. P. ( 2006; ). The genus Flavobacterium. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 481–531. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  3. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  4. Bernardet, J. F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  5. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  6. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  8. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  9. Hugh, R. & Leifson, E. ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66, 24–26.
    [Google Scholar]
  10. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  13. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  14. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  15. Qu, J.-H., Li, H.-F., Yang, J.-S. & Yuan, H.-L. ( 2008; ). Flavobacterium cheniae sp. nov., isolated from sediment of a eutrophic reservoir. Int J Syst Evol Microbiol 58, 2186–2190.[CrossRef]
    [Google Scholar]
  16. Reichenbach, H. ( 1992; ). The order Cytophagales. In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  19. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  20. Tamaki, H., Hanada, S., Kamagata, Y., Nakamura, K., Nomura, N., Nakano, K. & Matsumura, M. ( 2003; ). Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int J Syst Evol Microbiol 53, 519–526.[CrossRef]
    [Google Scholar]
  21. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2005; ). Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55, 769–772.[CrossRef]
    [Google Scholar]
  22. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  23. Zhang, D.-C., Wang, H.-X., Liu, H.-C., Dong, X.-Z. & Zhou, P.-J. ( 2006; ). Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 56, 2921–2925.[CrossRef]
    [Google Scholar]
  24. Zhu, F., Wang, S. & Zhou, P. ( 2003; ). Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53, 853–857.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007906-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007906-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error