1887

Abstract

Four Gram-positive, non-motile, aerobic actinobacteria were isolated from spiders and their webs. Their genetic, phenotypic and chemical properties were studied. The 16S rRNA gene sequence data suggested that the four novel isolates belonged to the genus . Two strains (FA1 and FA2) formed a cluster together with and and the other two strains (FB1 and FB2) formed a cluster together with and . The cell-wall peptidoglycan contained -Apm and mycolic acids were absent. Isoprenoid quinones were mainly composed of MK-9(H4), MK-9(H2) and MK-9 and the predominant fatty acids were 12-methyltetradecanoic acid (ai-C) and 13-methyltetradecanoic acid (i-C). The major polar lipids were phosphatidylinositol and phosphatidylglycerol. In addition, strain FA1, FB1, and FB2 contained diphosphatidylglycerol and phosphatidylcholine. The DNA G+C contents were: 72 mol%, 73 mol%, 74 mol% and 75 mol% for strains FA1, FA2, FB1, and FB2, respectively. DNA–DNA hybridization studies demonstrated that the novel strains showed low relatedness values to , , and . These data support the proposal that strains FA1, FA2, FB1 and FB2 represent novel species of the genus . Therefore, the names (type strain FA1=DSM 21741=NBRC 104963), (type strain FA2=DSM 21742=NBRC 104964), (type strain FB1=DSM 21744=NBRC 104966) and (type strain FB2=DSM 21743=NBRC 104965) are proposed for these new strains.

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2010-01-01
2019-10-21
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vol. , part 1, pp. 113 - 120

Polar lipid properties of the spider isolates and comparison with those of recognized species of the genus . [ PDF] 104 KB



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