1887

Abstract

We examined American Type Culture Collection (ATCC) strains of and by using multilocus DNA target sequencing. Apart from the type strain, all 10 ATCC strains examined were classified incorrectly, with most being reclassified as belonging to the ’ relatedness group. All four strains were tightly clustered, but heterogeneity was observed within the cluster. As a result of the incorrect annotation of the strains, two commonly used methods of identification are compromised and two case reports implicating as a human pathogen are probably incorrect, with the isolates probably belonging to the ’ relatedness group. These findings together with a review of isolates identified at two large reference laboratories suggest that is not a clinically significant isolate.

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2009-06-01
2024-04-19
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References

  1. Adékambi, T., Colson, P. & Drancourt, M.(2003).rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. J Clin Microbiol 41, 5699–5708.[CrossRef] [Google Scholar]
  2. Adékambi, T., Reynaud-Gaubert, M., Greub, G., Gevaudan, M. J., La Scola, B., Raoult, D. & Drancourt, M.(2004). Amoebal coculture of “Mycobacterium massiliense” sp. nov. from the sputum of a patient with hemoptoic pneumonia. J Clin Microbiol 42, 5493–5501.[CrossRef] [Google Scholar]
  3. Adékambi, T., Berger, P., Raoult, D. & Drancourt, M.(2006a).rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov. Int J Syst Evol Microbiol 56, 133–143.[CrossRef] [Google Scholar]
  4. Adékambi, T., Raoult, D. & Drancourt, M.(2006b).Mycobacterium barrassiae sp. nov., a Mycobacterium moriokaense group species associated with chronic pneumonia. J Clin Microbiol 44, 3493–3498.[CrossRef] [Google Scholar]
  5. Butler, W. R. & Guthertz, L. S.(2001). Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species. Clin Microbiol Rev 14, 704–726.[CrossRef] [Google Scholar]
  6. Devallois, A., Goh, K. S. & Rastogi, N.(1997). Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species. J Clin Microbiol 35, 2969–2973. [Google Scholar]
  7. Esteban, J., Fernandez-Roblas, R., Roman, A., Molleja, A., Jimenez, M. S. & Soriano, F.(1998). Catheter-related bacteremia due to Mycobacterium aurum in an immunocompromised host. Clin Infect Dis 26, 496–497.[CrossRef] [Google Scholar]
  8. Hall, L., Doerr, K. A., Wohlfiel, S. L. & Roberts, G. D.(2003). Evaluation of the MicroSeq system for identification of mycobacteria by 16S ribosomal DNA sequencing and its integration into a routine clinical mycobacteriology laboratory. J Clin Microbiol 41, 1447–1453.[CrossRef] [Google Scholar]
  9. Heckman, G. A., Hawkins, C., Morris, A., Burrows, L. L. & Bergeron, C.(2004). Rapidly progressive dementia due to Mycobacterium neoaurum meningoencephalitis. Emerg Infect Dis 10, 924–927.[CrossRef] [Google Scholar]
  10. Kiska, D. L., Turenne, C. Y., Dubansky, A. S. & Domachowske, J. B.(2004). First case report of catheter-related bacteremia due to “Mycobacterium lacticola”. J Clin Microbiol 42, 2855–2857.[CrossRef] [Google Scholar]
  11. Koranyi, K. I. & Ranalli, M. A.(2003).Mycobacterium aurum bacteremia in an immunocompromised child. Pediatr Infect Dis J 22, 1108–1109.[CrossRef] [Google Scholar]
  12. Lan, R. & Reeves, P. R.(2001). When does a clone deserve a name? A perspective on bacterial species based on population genetics. Trends Microbiol 9, 419–424.[CrossRef] [Google Scholar]
  13. Maiden, M. C., Bygraves, J. A., Feil, E., Morelli, G., Russell, J. E., Urwin, R., Zhang, Q., Zhou, J., Zurth, K. & other authors(1998). Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A 95, 3140–3145.[CrossRef] [Google Scholar]
  14. Simmon, K. E., Croft, A. C. & Petti, C. A.(2006). Application of SmartGene IDNS software to partial 16S rRNA gene sequences for a diverse group of bacteria in a clinical laboratory. J Clin Microbiol 44, 4400–4406.[CrossRef] [Google Scholar]
  15. Simmon, K. E., Pounder, J. I., Greene, J. N., Walsh, F., Anderson, C. M., Cohen, S. & Petti, C. A.(2007). Identification of an emerging pathogen, Mycobacterium massiliense, by rpoB sequencing of clinical isolates collected in the United States. J Clin Microbiol 45, 1978–1980.[CrossRef] [Google Scholar]
  16. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  17. Telenti, A., Marchesi, F., Balz, M., Bally, F., Bottger, E. C. & Bodmer, T.(1993). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31, 175–178. [Google Scholar]
  18. Tsukamura, M. & Mizuno, S.(1972). A new species of rapidly growing scotochromogenic mycobacteria. Mycobacterium neoaurum. Med Biol (Tokyo) 85, 229–233. [Google Scholar]
  19. Tsukamura, M. & Tsukamura, S.(1966).Mycobacterium aurum; a new species. Igaku To Seibutsugaku 72, 270–273. [Google Scholar]
  20. Washer, L. L., Riddell, J., Rider, J. & Chenoweth, C. E.(2007).Mycobacterium neoaurum bloodstream infection: report of 4 cases and review of the literature. Clin Infect Dis 45, e10–e13.[CrossRef] [Google Scholar]
  21. Williams, K. J., Ling, C. L., Jenkins, C., Gillespie, S. H. & McHugh, T. D.(2007). A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory. J Med Microbiol 56, 598–602.[CrossRef] [Google Scholar]
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Neighbour-joining trees based on and gene sequences. [PDF](21 KB)

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ATCC strains used and GenBank accession numbers for the genes investigated in this study. [PDF](77 KB)

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