1887

Abstract

Two ustilaginomycetous anamorphic strains were isolated from flowers in Taiwan. Phylogenetic analysis based on the combined rRNA gene sequence of internal transcribed spacer 1 (ITS1)–5.8S–ITS2 and large-subunit D1/D2 domains indicated that the closest recognized species was . The results of DNA–DNA hybridization and physiological characteristics showed that the two strains represent a novel species within the genus . The name sp. nov. is proposed, with FIRDI 005 (=BCRC 34227 =CBS 10937) as the type strain.

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2009-07-01
2020-01-29
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References

  1. Boekhout, T. ( 1995; ). Pseudozyma Bandoni emend. Boekhout, a genus for yeast-like anamorphs of Ustilaginales. J Gen Appl Microbiol 41, 359–366.[CrossRef]
    [Google Scholar]
  2. Boekhout, T. & Fell, J. W. ( 1998; ). Pseudozyma Bandoni emend. Boekhout and a comparison with the yeast state of Ustilago maydis (De Candolle) Corda. In The Yeasts, a Taxonomic Study, 4th edn, pp. 790–797. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  3. Boekhout, T., Bandoni, R. J., Fell, J. W. & Kwon-Chung, K. J. ( 1998; ). Discussion of teleomorphic and anamorphic genera of heterobasidiomycetous yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 609–625. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  4. Fell, J. W., Boekhout, T., Fonseca, A., Scorzetti, G. & Statzell-Tallman, A. ( 2000; ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50, 1351–1371.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 2004; ). phylip (phylogenetic inference package), version 3.6. Distributed by the author. Department of Genome Sciences and Department of Biology, University of Washington, Seattle, WA, USA.
  6. Golubev, W., Sugita, T. & Golubev, N. ( 2007; ). An ustilaginomycetous yeast, Pseudozyma graminicola sp. nov., isolated from the leaves of pasture plants. Mycoscience 48, 29–33.[CrossRef]
    [Google Scholar]
  7. Lee, F.-L., Lee, C.-F., Okada, S., Uchimura, T., Komagata, K. & Kozaki, M. ( 1993; ). Candida galacta comb. nov., a new combination for Candida apis var. galacta. Int J Syst Bacteriol 43, 183–184.[CrossRef]
    [Google Scholar]
  8. Lee, F.-L., Fu, H.-M. & Hsu, W.-H. ( 1998; ). DNA hybridization and electrokaryotype study of some Candida species. Int J Syst Bacteriol 48, 1463–1466.[CrossRef]
    [Google Scholar]
  9. O'Donnell, K. ( 1992; ). Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum (Gibberella pulicaris). Curr Genet 22, 213–220.[CrossRef]
    [Google Scholar]
  10. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  11. Seo, H.-S., Um, H.-J., Min, J., Rhee, S.-K., Cho, T.-J., Kim, Y.-H. & Lee, J. ( 2007; ). Pseudozyma jejuensis sp. nov., a novel cutinolytic ustilaginomycetous yeast species that is able to degrade plastic waste. FEMS Yeast Res 7, 1035–1045.[CrossRef]
    [Google Scholar]
  12. Stoll, M., Begerow, D. & Oberwinkler, F. ( 2005; ). Molecular phylogeny of Ustilago, Sporisorium, and related taxa based on combined analysis of rDNA sequences. Mycol Res 109, 342–356.[CrossRef]
    [Google Scholar]
  13. Sugita, T., Nishikawa, A., Ikeda, R. & Shinoda, T. ( 1999; ). Identification of medically relevant Trichosporon species based on sequences of internal transcribed spacer regions and construction of a database for Trichosporon identification. J Clin Microbiol 37, 1985–1993.
    [Google Scholar]
  14. Sugita, T., Takashima, M., Poonwan, N., Mekha, N., Malaithao, K., Thungmuthasawat, B., Prasarn, S., Luangsook, P. & Kudo, T. ( 2003; ). The first isolation of ustilaginomycetous anamorphic yeasts, Pseudozyma species, from patients' blood and a description of two new species: P. parantarctica and P. thailandica. Microbiol Immunol 47, 183–190.[CrossRef]
    [Google Scholar]
  15. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  17. Wang, Q.-M., Jia, J.-H. & Bai, F.-Y. ( 2006; ). Pseudozyma hubeiensis sp. nov. and Pseudozyma shanxiensis sp. nov., novel ustilaginomycetous anamorphic yeast species from plant leaves. Int J Syst Evol Microbiol 56, 289–293.[CrossRef]
    [Google Scholar]
  18. Wei, Y.-H., Lee, F.-L., Hsu, W.-H., Chen, S.-R., Chen, C.-C., Wen, C.-Y., Lin, S.-J., Chu, W.-S., Yuan, G.-F. & Liou, G.-Y. ( 2005; ). Pseudozyma antarctica in Taiwan: a description based on morphological, physiological and molecular characteristics. Bot Bull Acad Sinica (Taiwan) 46, 223–229.
    [Google Scholar]
  19. White, T. J., Bruns, T., Lee, S. & Taylor, J. ( 1990; ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications, pp. 315–322. Edited by M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White. San Diego: Academic Press.
  20. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
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Maximum-parsimony tree of the genus and related teleomorphs, based on the combined sequences of the ITS and LSU rRNA gene D1/D2 domain [ PDF] (182 KB)

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DNA base composition and DNA relatedness values between sp. nov. and closely related species [ PDF] (33 KB)

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