1887

Abstract

A slightly curved, rod-shaped marine bacterium, designated strain CL-S1, was isolated from near Dokdo, an island in the East Sea, Korea. Cells were Gram-negative and grew well under either aerobic or microaerobic conditions. Analyses of the 16S rRNA and gene sequences of strain CL-S1 revealed an affiliation with the genus within the class . Phylogenetic analyses based on 16S rRNA and gene sequences showed that strain CL-S1 formed a robust clade with LA31B, with sequence similarities of 96.1 and 88.2 %, respectively. DNA–DNA relatedness between strain CL-S1 and DSM 18005 was 44 %, indicating that they represent genomically distinct species. Strain CL-S1 grew optimally at 30–37 °C, at pH 7 and in the presence of 3–5 % NaCl. The dominant cellular fatty acids were iso-C 2-OH and/or C 7 (28.4 %), C (26.2 %) and C 7 (22.3 %). The DNA G+C content of strain CL-S1 was 28 mol%. Strain CL-S1 differed phenotypically from LA31B based on its ability to grow aerobically at 10 °C and inability to grow under anaerobic conditions. Based on the data presented, strain CL-S1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CL-S1 (=KCCM 90072 =JCM 15502).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007740-0
2010-03-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/531.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007740-0&mimeType=html&fmt=ahah

References

  1. Abdelbaqi, K., Ménard, A., Prouzet-Mauleon, V., Bringaud,F., Lehours, P. & Mégraud, F. ( 2007; ). Nucleotidesequence of the gyrA gene of Arcobacter species and characterizationof human ciprofloxacin-resistant clinical isolates. FEMS ImmunolMed Microbiol 49, 337–345.
    [Google Scholar]
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman,D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  3. Anzai, Y., Kudo, Y. & Oyaizu, H. ( 1997; ). The phylogeny of the genera Chryseomonas, Flavimonas,and Pseudomonas supports synonymy of these three genera. Int J Syst Bacteriol 47, 249–251.[CrossRef]
    [Google Scholar]
  4. Atabay, H. I., Corry, J. E. L. & On, S. L. W. ( 1998; ). Diversity and prevalence of Arcobacter spp. inbroiler chickens. J Appl Microbiol 84, 1007–1016.[CrossRef]
    [Google Scholar]
  5. Bouchotroch, S., Quesada, E., del Moral, A., Llamas, I. &Béjar, V. ( 2001; ). Halomonas maurasp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 51, 1625–1632.[CrossRef]
    [Google Scholar]
  6. Choi, D. H., Kim, Y.-G., Hwang, C. Y., Yi, H., Chun, J. &Cho, B. C. ( 2006; ). Tenacibaculum litoreumsp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 635–640.[CrossRef]
    [Google Scholar]
  7. Cole, J. R., Chai, B., Farris, R. J., Wang, Q., Kulam-Syed-Mohideen,A. S., McGarrell, D. M., Bandela, A. M., Cardenas, E., Garrity, G. M. &other authors ( 2007; ). The ribosomal database project (RDP-II):introducing myRDP space and quality controlled public data. Nucleic Acids Res 35, D169–D172.[CrossRef]
    [Google Scholar]
  8. Donachie, S. P., Bowman, J. P., On, S. L. W. & Alam, M. ( 2005; ). Arcobacter halophilus sp. nov.,the first obligate halophile in the genus Arcobacter. Int J Syst Evol Microbiol 55, 1271–1277.[CrossRef]
    [Google Scholar]
  9. Englen, M. D. & Kelley, L. C. ( 2000; ). A rapid DNA isolation procedure for the identification of Campylobacterjejuni by the polymerase chain reaction. Lett Appl Microbiol 31, 421–426.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1981; ). Evolutionarytrees from DNA sequences: a maximum likelihood approach. J MolEvol 17, 368–376.
    [Google Scholar]
  11. Fitch, W. M. ( 1971; ). Toward definingthe course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  12. Hansen, G. H. & Sørheim, R. ( 1991; ). Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13, 231–241.[CrossRef]
    [Google Scholar]
  13. Houf, K., On, S. L. W., Coenye, T., Mast, J., Hoof, J. V. &Vandamme, P. ( 2005; ). Arcobacter cibariussp. nov., isolated from broiler carcasses. Int J Syst Evol Microbiol 55, 713–717.[CrossRef]
    [Google Scholar]
  14. Jeon, Y.-S., Chung, H., Park, S., Hur, I., Lee, J.-H. &Chun, J. ( 2005; ). jPHYDIT: a JAVA-based integratedenvironment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21, 3171–3173.[CrossRef]
    [Google Scholar]
  15. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism,vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  16. Kiehlbauch, J. A., Brenner, D. J., Nicholson, M. A., Baker,C. N., Patton, C. M., Steigerwalt, A. G. & Wachsmuth, I. K. ( 1991; ). Campylobacter butzleri sp. nov. isolated from humansand animals with diarrheal illness. J Clin Microbiol 29, 376–385.
    [Google Scholar]
  17. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega 3: integrated software for molecular evolutionary geneticsanalysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  18. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D.H., Lim, C. K. & Go, S. J. ( 2003; ). Pseudomonaskoreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  19. Lane, D. J. ( 1991; ). 16S/23S rRNAsequencing. In Nucleic Acid Techniques in Bacterial Systematics,pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester:Wiley.
  20. Mansfield, L. P. & Forsythe, S. J. ( 2000; ). Arcobacter butzleri, A. skirrowii and A. cryaerophilus – potential emerging human pathogens. Rev Med Microbiol 11, 161–170.[CrossRef]
    [Google Scholar]
  21. Marmur, J. ( 1961; ). A procedure for theisolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  22. McClung, C. R., Patriquin, D. G. & Davis, R. E. ( 1983; ). Campylobacter nitrofigilis sp. nov., a nitrogen-fixingbacterium associated with roots of Spartina alterniflora. Int J Syst Bacteriol 33, 605–612.[CrossRef]
    [Google Scholar]
  23. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleicacid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  24. Neill, S. D., Campbell, J. N., O'Brien, J. J., Weatherup,S. T. C. & Ellis, W. A. ( 1985; ). Taxonomic positionof Campylobacter cryaerophila sp. nov. Int J Syst Bacteriol 35, 342–356.[CrossRef]
    [Google Scholar]
  25. Notredame, C., Higgins, D. G. & Heringa, J. ( 2000; ). T-Coffee: a novel method for fast and accurate multiplesequence alignment. J Mol Biol 302, 205–217.[CrossRef]
    [Google Scholar]
  26. On, S. L. W., Holmes, B. & Sackin, M. J. ( 1996; ). A probability matrix for the identification of campylobacters,helicobacters and allied taxa. J Appl Bacteriol 81, 425–432.
    [Google Scholar]
  27. Owen, R. J., Martin, S. R. & Borman, P. ( 1985; ). Rapid urea hydrolysis by gastric campylobacters. Lancet 1, 111
    [Google Scholar]
  28. Popovic-Uroic, T., Patton, C. M., Nicholson, M. A. & Kiehlbauch,J. A. ( 1990; ). Evaluation of the indoxyl acetate hydrolysistest for rapid differentiation of Campylobacter, Helicobacter, and Wolinella species. J Clin Microbiol 28, 2335–2339.
    [Google Scholar]
  29. Posada, D. & Crandall, K. A. ( 1998; ). MODELTEST: testing the model of DNA substitution. Bioinformatics 14, 817–818.[CrossRef]
    [Google Scholar]
  30. Reddy, C. A., Beveridge, T. J., Brenznak, J. A., Marzluf, G.A., Schmidt, T. M. & Snyder, L. R. ( 2007; ). In Methods for General and Molecular Microbiology, 3rd edn, section VI,p. 957. Washington, DC: American Society for Microbiology.
  31. Rozen, S. & Skaletsky, H. ( 2000; ).Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics Methods and Protocols: Methods in Molecular Biology, pp. 365–386. Edited by S. Krawetz & S. Misener. Totowa, NJ:Humana Press.
  32. Saitou, N. & Nei, M. ( 1987; ). Theneighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  33. Skirrow, M. B. & Benjamin, J. ( 1980; ). Differentiation of enteropathogenic Campylobacter. J Clin Pathol 33, 1122 [CrossRef]
    [Google Scholar]
  34. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and MolecularBacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray,W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  35. Swofford, D. L. ( 2002; ). paup*:Phylogenetic analysis using parsimony (and other methods), version4. Sunderland, MA: Sinauer Associates.
  36. Teske, A., Sigalevich, P., Cohen, Y. & Muyzer, G. ( 1996; ). Molecular identification of bacteria from a cocultureby denaturing gradient gel electrophoresis of 16S ribosomal DNA fragmentsas a tool for isolation in pure cultures. Appl Environ Microbiol 62, 4210–4215.
    [Google Scholar]
  37. Vandamme, P., Falsen, E., Rossau, R., Hoste, B., Segers, P.,Tytgat, R. & De Ley, J. ( 1991; ). Revision of Campylobacter, Helicobacter, and Wolinella taxonomy:emendation of generic descriptions and proposal of Arcobacter gen.nov. Int J Syst Bacteriol 41, 88–103.[CrossRef]
    [Google Scholar]
  38. Vandamme, P., Vancanneyt, M., Pot, B., Mels, L., Hoste, B.,Dewettinck, D., Vlaes, L., Van Den Borre, C., Higgins, R. & other authors ( 1992; ). Polyphasic taxonomic study of the emendedgenus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated fromveterinary specimens. Int J Syst Bacteriol 42, 344–356.[CrossRef]
    [Google Scholar]
  39. Vandamme, P., Dewhirst, F. E., Paster, B. J. & On, S. L.W. ( 2005a; ). Family I. CampylobacteraceaeVandamme and De Ley 1991, 453VP. In Bergey'sManual ofSystematic Bacteriology, 2nd edn, vol. 2, part C, pp. 1145–1146.Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. NewYork: Springer.
  40. Vandamme, P., Dewhirst, F. E., Paster, B. J. & On, S. L.W. ( 2005b; ). Genus II. Arcobacter Vandamme,Falsen, Rossau, Hoste, Segers, Tytgat and De Ley 1991a, 99VP emend.Vandamme, Falsen, Rossau, Hoste, Dewettinck, Vlaes, Van Den Borre, Higgins,Hommez, Kersters, Butzler and Goossens 1992b, 355. In Bergey'sManualof Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 1161–1165.Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. NewYork: Springer.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007740-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007740-0
Loading

Data & Media loading...

Supplements

Neighbour-joining trees based on gene sequences and inferred amino acid sequences of the gene, showing the relationships between strain CL-S1 and members of the genus . [PDF](42 KB)

PDF

Transmission electron micrographs of negatively stained cells of strain CL-S1 grown on blood agar at 37 °C for 1 day. Bars, 0.5 µm (left) and 2 µm (right).

IMAGE

Transmission electron micrographs of negatively stained cells of strain CL-S1 grown on blood agar at 37 °C for 1 day. Bars, 0.5 µm (left) and 2 µm (right).

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error