1887

Abstract

A novel Gram-positive-staining actinomycete designated strain GMKU095 was isolated from surface-sterilized roots of A. Cunn. ex Benth. (earpod wattle). The organism produced branching mycelium. The spores were non-motile and had a spiny surface. Growth of strain GMKU095 occurred at 18–42 °C, pH 5.0–8.0 and at NaCl concentrations up to 5 %. Whole-cell hydrolysates contained arabinose and galactose as major characteristic sugars. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The glycan moiety of the murein contained acetyl residues. The menaquinone was MK-8(H); mycolic acids were not detected. The G+C content of the DNA was 71.6 mol%. iso-C was detected as the major cellular fatty acid. Comparative studies of 16S rRNA gene sequences indicated that the strain was phylogenetically related to members of the genus . The most closely related type strain is IMSNU 50581, which is 96.2 % similar in 16S rRNA gene sequence. On the basis of the genotypic and phenotypic properties presented, a novel species of the genus is proposed, sp. nov. The type strain is strain GMKU095 (=NRRL B-24609 =BCC 28481 =TISTR 1862 =NBRC 104274).

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2009-06-01
2020-01-29
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References

  1. Becker, B., Lechevalier, M. P. & Lechevalier, H. A. ( 1965; ). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13, 236–243.
    [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  3. Duangmal, K., Ward, A. C. & Goodfellow, M. ( 2005; ). Selective isolation of members of the Streptomyces violaceoruber clade from soil. FEMS Microbiol Lett 245, 321–327.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  7. Gordon, R. E. & Mihm, J. M. ( 1957; ). A comparative study of some strains received as nocardiae. J Bacteriol 73, 15–27.
    [Google Scholar]
  8. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  9. Gu, Q., Luo, H., Zheng, W., Liu, Z. & Huang, Y. ( 2006; ). Pseudonocardia oroxyli sp. nov., a novel actinomycete isolated from surface-sterilized Oroxylum indicum root. Int J Syst Evol Microbiol 56, 2193–2197.[CrossRef]
    [Google Scholar]
  10. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  11. Henssen, A. ( 1957; ). Beiträge zur Morphologie und Systematik der thermophilen Actinomyceten. Arch Mikrobiol 26, 373–414 (in German).[CrossRef]
    [Google Scholar]
  12. Huang, Y., Wang, L., Lu, Z., Hong, L., Liu, Z., Tan, G. Y. A. & Goodfellow, M. ( 2002; ). Proposal to combine the genera Actinobispora and Pseudonocardia in an emended genus Pseudonocardia, and description of Pseudonocardia zijingensis sp. nov. Int J Syst Evol Microbiol 52, 977–982.[CrossRef]
    [Google Scholar]
  13. Kämpfer, P., Kohlweyer, U., Thiemer, B. & Andreesen, J. R. ( 2006; ). Pseudonocardia tetrahydrofuranoxydans sp. nov. Int J Syst Evol Microbiol 56, 1535–1538.[CrossRef]
    [Google Scholar]
  14. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  15. Küster, E. & Williams, S. T. ( 1964; ). Media for the isolation of streptomycetes: starch casein medium. Nature 202, 928–929.[CrossRef]
    [Google Scholar]
  16. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  17. Lee, S. D., Kim, E. S., Min, K. L., Lee, W. Y., Kang, S. O. & Hah, Y. C. ( 2001; ). Pseudonocardia kongjuensis sp. nov., isolated from a gold mine cave. Int J Syst Evol Microbiol 51, 1505–1510.
    [Google Scholar]
  18. Lee, S. D., Kim, E. S., Kang, S. O. & Hah, Y. C. ( 2002; ). Pseudonocardia spinosispora sp. nov., isolated from Korean soil. Int J Syst Evol Microbiol 52, 1603–1608.[CrossRef]
    [Google Scholar]
  19. Liu, Z. P., Wu, J. F., Liu, Z. H. & Liu, S. J. ( 2006; ). Pseudonocardia ammonioxydans sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 56, 555–558.[CrossRef]
    [Google Scholar]
  20. Mahendra, S. & Alvarez-Cohen, L. ( 2005; ). Pseudonocardia dioxanivorans sp. nov., a novel actinomycete that grows on 1,4-dioxane. Int J Syst Evol Microbiol 55, 593–598.[CrossRef]
    [Google Scholar]
  21. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  22. McVeigh, H. P., Munro, J. & Embley, T. M. ( 1994; ). The phylogenetic position of Pseudoamycolata halophobica (Akimov et al. 1989) and a proposal to reclassify it as Pseudonocardia halophobica. Int J Syst Bacteriol 44, 300–302.[CrossRef]
    [Google Scholar]
  23. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  24. Reichert, K., Lipski, A., Pradella, S., Stackebrandt, E. & Altendorf, K. ( 1998; ). Pseudonocardia asaccharolytica sp. nov. and Pseudonocardia sulfidoxydans sp. nov., two new dimethyl disulfide-degrading actinomycetes and emended description of the genus Pseudonocardia. Int J Syst Bacteriol 48, 441–449.[CrossRef]
    [Google Scholar]
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  26. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  27. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  28. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  29. Tamaoka, J., Katayara-Fujimura, Y. & Kuraishi, H. ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef]
    [Google Scholar]
  30. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  31. Tomiyasu, I. ( 1982; ). Mycolic acid composition and thermally adaptative changes in Nocardia asteroides. J Bacteriol 151, 828–837.
    [Google Scholar]
  32. Uchida, K. & Aida, K. ( 1984; ). An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef]
    [Google Scholar]
  33. Warwick, S., Bowen, T., McVeigh, H. P. & Embley, T. M. ( 1994; ). A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia. Int J Syst Bacteriol 44, 293–299.[CrossRef]
    [Google Scholar]
  34. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
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