1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 6

sp. nov., isolated from roots of A. Cunn. ex Benth. Free

Abstract

A novel Gram-positive-staining actinomycete designated strain GMKU095 was isolated from surface-sterilized roots of A. Cunn. ex Benth. (earpod wattle). The organism produced branching mycelium. The spores were non-motile and had a spiny surface. Growth of strain GMKU095 occurred at 18–42 °C, pH 5.0–8.0 and at NaCl concentrations up to 5 %. Whole-cell hydrolysates contained arabinose and galactose as major characteristic sugars. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The glycan moiety of the murein contained acetyl residues. The menaquinone was MK-8(H); mycolic acids were not detected. The G+C content of the DNA was 71.6 mol%. iso-C was detected as the major cellular fatty acid. Comparative studies of 16S rRNA gene sequences indicated that the strain was phylogenetically related to members of the genus . The most closely related type strain is IMSNU 50581, which is 96.2 % similar in 16S rRNA gene sequence. On the basis of the genotypic and phenotypic properties presented, a novel species of the genus is proposed, sp. nov. The type strain is strain GMKU095 (=NRRL B-24609 =BCC 28481 =TISTR 1862 =NBRC 104274).

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Keyword(s): A2pm, diaminopimelic acid
© SGM
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2009-06-01
2024-04-23
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Pseudonocardia acaciae sp. nov., isolated from roots of Acacia auriculiformis A. Cunn. ex Benth.
Int J Syst Evol Microbiol 59, 1487 (2009); https://doi.org/10.1099/ijs.0.007724-0
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