Two novel halophilic, aerobic, catalase-positive but oxidase-negative, Gram-positive actinomycetes, designated YIM 91353 and YIM 91394, were isolated from a salt lake in the north-west of China. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the novel isolates should be assigned to the genus . The phenotypic and chemotaxonomic characteristics of the isolates also matched those described for members of the genus . The predominant menaquinones were MK-10(H), MK-10(H) and MK-9(H), and -diaminopimelic acid was the diagnostic amino acid in the cell walls. The phospholipids of the isolates consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major fatty acids of strain YIM 91353 were anteiso-C and C, and of strain YIM 91394 were anteiso-C and iso-C. The DNA G+C contents were 71.2 and 72.5 mol%, respectively. The combination of phylogenetic analysis, DNA–DNA hybridization data, phenotypic characteristics and chemotaxonomic differences supported the view that strains YIM 91353 and YIM 91394 each represent a novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, with type strains YIM 91353 (=DSM 45171=CCTCC AA 208048) and YIM 91394 (=DSM 45155=CCTCC AA 208047), respectively.


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Minimum-evolution and maximum-parsimony 16S rRNA gene sequence phylogenies of strains YIM 91353 ( sp. nov.) and YIM 91394 ( sp. nov. [PDF](19 KB)


Fatty acid compositions of strains YIM 91353 ( sp. nov.) and YIM 91394 ( sp. nov.) and other members of the genus [PDF](22 KB)

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