1887

Abstract

Two strictly anaerobic, crescent-shaped bacterial strains, designated WG and Ycb08, were isolated from a cellulose-degrading mixed culture enriched from yak rumen contents. The strains were Gram-negative, non-spore-forming and motile, with four to six flagella situated at the centre of the concave side of the cell. The cells were 0.9–1.1×4–6 μm. Growth was observed at 27–46 °C (optimum 39 °C) and pH 4.2–8.3 (optimum pH 7.0–7.2). Arabinose, glucose, mannose, cellobiose, lactose, sucrose, trehalose, melibiose, raffinose, salicin and aesculin were fermented. The end products of glucose fermentation were acetate, propionate and CO. The G+C contents of strains WG and Ycb08 were respectively 63.9±0.2 and 62.5±0.2 mol% ( ). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains were related to the genera and at similarity levels below 97 %; however, they differed from members of the genus in their flagellar arrangement. On the basis of phenotypic, genotypic and physiological evidence, strains WG and Ycb08 are identified as members of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WG (=CGMCC 1.5073 =JCM 15470).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007641-0
2009-08-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/8/2080.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007641-0&mimeType=html&fmt=ahah

References

  1. An, D., Dong, X. & Dong, Z. ( 2005; ). Prokaryote diversity in the rumen of yak (Bos grunniens) and Jinnan cattle (Bos taurus) estimated by 16S rDNA homology analyses. Anaerobe 11, 207–215.[CrossRef]
    [Google Scholar]
  2. Bryant, M. P. ( 1956; ). The characteristics of strains of Selenomonas isolated from bovine rumen contents. J Bacteriol 72, 162–167.
    [Google Scholar]
  3. Bryant, M. P. ( 1984; ). Genus IX. Selenomonas Von Prowazek 1913, 36AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 650–653. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Holdeman, L. V., Cato, E. P. & Moore, M. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute & State University.
  6. Holdeman, L. V., Kelley, R. W. & Moore, W. E. C. ( 1984; ). Genus I. Bacteroides Castellani and Chalmers 1919, 959AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 604–631. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  7. Hungate, R. E. ( 1966; ). The Rumen and its Microbes. New York: Academic Press.
  8. Hungate, R. E. ( 1969; ). A roll-tube method for cultivation of strict anaerobes. Methods Microbiol 3B, 117–132.
    [Google Scholar]
  9. Kingsley, V. V. & Hoeniger, J. F. ( 1973; ). Growth, structure and classification of Selenomonas. Bacteriol Rev 37, 479–521.
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Lan, G. Q., Ho, Y. W. & Abdullah, N. ( 2002; ). Mitsuokella jalaludinii sp. nov., from the rumens of cattle in Malaysia. Int J Syst Evol Microbiol 52, 713–718.[CrossRef]
    [Google Scholar]
  12. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  13. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  14. Mitsuoka, T., Terada, A., Watanabe, K. & Uchida, K. ( 1974; ). Bacteroides multiacidus, a new species from the feces of humans and pigs. Int J Syst Bacteriol 24, 35–41.[CrossRef]
    [Google Scholar]
  15. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  16. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  17. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007641-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007641-0
Loading

Data & Media loading...

Supplements

vol. , part 8, pp. 2080 - 2083

Transmission electron micrograph of negatively stained cell of sp. nov. WG . The tuft of flagella is shown. Bar, 1 µm.



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error