RT Journal Article SR Electronic(1) A1 Yoon, Jung-Hoon A1 Schumann, Peter A1 Kang, So-Jung A1 Lee, Chang-Soo A1 Lee, Soo-Young A1 Oh, Tae-KwangYR 2009 T1 Microbacterium insulae sp. nov., isolated from soil JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 7 SP 1738 OP 1742 DO https://doi.org/10.1099/ijs.0.007591-0 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, non-motile, rod- or coccoid-shaped Microbacterium-like bacterium, designated strain DS-66T, was isolated from soil of Dokdo, Korea, and its exact taxonomic position was investigated by using a polyphasic approach. Strain DS-66T grew optimally at 30 °C and pH 6.5–7.0 in the presence of 0.5–1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-66T belonged to the genus Microbacterium. Strain DS-66T had a peptidoglycan type based on B2β with partial substitution of glutamic acid by 3-hydroxy glutamic acid (Glu/Hyg–Gly–d-Orn), and galactose, rhamnose and ribose as whole-cell sugars. The acyl type was glycolyl. Strain DS-66T contained MK-13, MK-12 and MK-14 as predominant menaquinones and anteiso-C15 : 0, anteiso-C17 : 0, iso-C17 : 0 and iso-C16 : 0 as major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. The DNA G+C content was 69.9 mol%. Phylogenetic distinctiveness, DNA–DNA relatedness data and differential phenotypic properties demonstrated that strain DS-66T is distinguishable from recognized Microbacterium species. On the basis of the data presented, strain DS-66T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium insulae sp. nov. is proposed. The type strain is DS-66T (=KCTC 19247T=CCUG 54523T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.007591-0