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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 7

sp. nov., isolated from soil Free

Abstract

A Gram-positive, non-motile, rod- or coccoid-shaped -like bacterium, designated strain DS-66, was isolated from soil of Dokdo, Korea, and its exact taxonomic position was investigated by using a polyphasic approach. Strain DS-66 grew optimally at 30 °C and pH 6.5–7.0 in the presence of 0.5–1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-66 belonged to the genus . Strain DS-66 had a peptidoglycan type based on B2 with partial substitution of glutamic acid by 3-hydroxy glutamic acid (Glu/Hyg–Gly–-Orn), and galactose, rhamnose and ribose as whole-cell sugars. The acyl type was glycolyl. Strain DS-66 contained MK-13, MK-12 and MK-14 as predominant menaquinones and anteiso-C, anteiso-C, iso-C and iso-C as major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. The DNA G+C content was 69.9 mol%. Phylogenetic distinctiveness, DNA–DNA relatedness data and differential phenotypic properties demonstrated that strain DS-66 is distinguishable from recognized species. On the basis of the data presented, strain DS-66 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-66 (=KCTC 19247=CCUG 54523).

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2009-07-01
2025-07-12
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Microbacterium insulae sp. nov., isolated from soil
Int J Syst Evol Microbiol 59, 1738 (2009); https://doi.org/10.1099/ijs.0.007591-0
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vol. , part 7, pp. 1738 - 1742

Extended neighbour-joining tree based on 16S rRNA gene sequences. [PDF](22 KB)

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