1887

Abstract

Ten strains, previously isolated from different Bobal grape wines from the Utiel-Requena Origin Denomination of Spain, were characterized phylogenetically, genotypically and phenotypically. The 16S rRNA genes were sequenced and phylogenetic analysis showed that they form a tight phylogenetic clade that is closely related to reference strains NRIC 0604, ‘’ 8 and DSM 20444. DNA–DNA hybridization results confirmed the separation of the strains from other species. Genotypically, the strains could be differentiated from their closest neighbours by 16S amplified rDNA restriction analysis and random amplified polymorphic DNA patterns. The strains were Gram-staining-positive, facultatively anaerobic rods that did not exhibit catalase activity. Phenotypically, they could be distinguished from their closest relatives by several traits such as their inabilities to grow at pH 3.3, to ferment sucrose, amygdalin and arbutin or to hydrolyse aesculin. The characteristics of the ten wine isolates suggest that they represent a novel species, for which the name sp. nov. is proposed. The type strain is 59b (=CECT 7334 =DSM 19972).

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2009-08-01
2020-01-27
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Phylogenetic trees based on 16S rRNA gene sequences and constructed using the maximum-parsimony method (Fig. S1; top) and the neighbour-joining method (Fig. S2; bottom). Bootstrap values (≥50%) based on 1000 resamplings are shown at branch nodes. The sequence of DSM 20252 was used as the outgroup. Bar, 10 % nucleotide sequence divergence.

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