1887

Abstract

An endophytic actinomycete, strain YIM 65188, was isolated from surface-sterilized tissue of sp. collected from Yunnan province, south-west China, and characterized by using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain YIM 65188 is a member of the genus and exhibited 97.5 and 96.3 % gene sequence similarities to GW41-1564 and YIM 47672, respectively, whereas low sequence similarity values (<95.2 %) distinguished strain YIM 65188 from all other species with validly published names. Strain YIM 65188 had a unique menaquinone composition, with the predominant quinones being MK-11(H) and MK-10(H), with moderate amounts of MK-10(H), MK-11(H), MK-9(H), MK-11(H), MK-9(H) and MK-10(H). Similarly, the closest phylogenetic relative, GW41-1564, also had an unusual quinone composition, with the predominant menaquinones MK-10(H) and MK-10(H) and minor amounts of MK-9(H) and MK-9(H). The DNA–DNA hybridization value between strain YIM 65188 and GW41-1564 was 42.7 %. On the basis of phenotypic and phylogenetic characteristics, strain YIM 65188 was identified as a novel species of the genus for which the name sp. nov. is proposed, with YIM 65188 (=CCTCC AA 208020 =DSM 41942) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007534-0
2009-06-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/6/1492.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007534-0&mimeType=html&fmt=ahah

References

  1. Atalan, E., Manfio, G. P., Ward, A. C., Kroppenstedt, R. M. & Goodfellow, M. ( 2000; ). Biosystematic studies on novel streptomycetes from soil. Antonie van Leeuwenhoek 77, 337–353.[CrossRef]
    [Google Scholar]
  2. Bérdy, J. ( 2005; ). Bioactive microbial metabolites, a personal view. J Antibiot (Tokyo) 58, 1–26.
    [Google Scholar]
  3. Collins, M. D. & Jones, D. ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  5. Coombs, J. T. & Franco, C. M. M. ( 2003; ). Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol 69, 5603–5608.[CrossRef]
    [Google Scholar]
  6. Dong, X. Z. & Cai, M. Y. ( 2001; ). Manual of Systematics and Identification of General Bacteria. Beijing: Science Press.
  7. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  9. Goodfellow, M. ( 1971; ). Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69, 33–80.[CrossRef]
    [Google Scholar]
  10. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strains. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  11. He, L., Li, W., Huang, Y., Wang, L. M., Liu, Z. H., Lanoot, B. J., Vancanneyt, M. & Swings, J. ( 2005; ). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55, 1939–1944.[CrossRef]
    [Google Scholar]
  12. Jiang, Y., Tang, S. K., Wiese, J., Xu, L. H., Imhoff, J. F. & Jiang, C. L. ( 2007; ). Streptomyces hainanensis sp. nov., a novel member of the genus Streptomyces. Int J Syst Evol Microbiol 57, 2694–2698.[CrossRef]
    [Google Scholar]
  13. Kämpfer, P., Huber, B., Buczolits, S., Thummes, K., Wollny, I. G. & Busse, H. J. ( 2008; ). Streptomyces specialis sp. nov. Int J Syst Evol Microbiol 58, 2602–2606.[CrossRef]
    [Google Scholar]
  14. Kelly, K. L. ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  15. Kim, S. B. & Goodfellow, M. ( 2002; ). Streptomyces thermospinisporus sp. nov., a moderately thermophilic caboxydotrophic streptomycete isolated from soil. Int J Syst Evol Microbiol 52, 1225–1228.[CrossRef]
    [Google Scholar]
  16. Kim, S. B., Lonsdale, J., Seong, C. N. & Goodfellow, M. ( 2003; ). Streptacidiphilus gen. nov., acidophilic actinomycetes with wall chemotype I and emendation of the family Streptomycetaceae (Waksman and Henrici (1943)AL) emend. Rainey et al. 1997. Antonie van Leeuwenhoek 83, 107–116.[CrossRef]
    [Google Scholar]
  17. Li, W., Lanoot, B., Zhang, Y., Vancanneyt, M., Swings, J. & Liu, Z. ( 2002; ). Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiated spore chains. Int J Syst Evol Microbiol 52, 1629–1633.[CrossRef]
    [Google Scholar]
  18. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L. ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef]
    [Google Scholar]
  19. Locci, R. ( 1989; ). Streptomyces and related genera. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2451–2508. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  20. Manfio, G. P., Zakrzewska-Czerwinska, J., Atalan, E. & Goodfellow, M. ( 1995; ). Towards minimal standards for the description of Streptomyces species. Biotechnologia 8, 228–237.
    [Google Scholar]
  21. Manfio, G. P., Atalan, E., Zakrzewska-Czerwinska, J., Mordarski, M., Rodriguez, C., Collins, M. D. & Goodfellow, M. ( 2003; ). Classification of novel soil streptomycetes as Streptomyces aureus sp. nov., Streptomyces laceyi sp. nov. and Streptomyces sanglieri sp. nov. Antonie Van Leeuwenhoek 83, 245–255.[CrossRef]
    [Google Scholar]
  22. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  23. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef]
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Sembiring, L., Ward, A. C. & Goodfellow, M. ( 2000; ). Selective isolation and characterization of members of the Streptomyces violaceusniger clade associated with roots of Paraserianthes falcataria. Antonie Van Leeuwenhoek 78, 353–366.[CrossRef]
    [Google Scholar]
  26. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  27. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  28. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H. ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef]
    [Google Scholar]
  29. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  30. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  31. Waksman, S. A. & Henrici, A. T. ( 1943; ). The nomenclature and classification of the actinomycetes. J Bacteriol 46, 337–341.
    [Google Scholar]
  32. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J. ( 1983; ). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813.
    [Google Scholar]
  33. Xu, P., Li, W. J., Tang, S. K., Zhang, Y. Q., Chen, G. Z., Chen, H. H., Xu, L. H. & Jiang, C. L. ( 2005; ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol 55, 1149–1153.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007534-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007534-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error