An endophytic actinomycete, strain YIM 65188, was isolated from surface-sterilized tissue of sp. collected from Yunnan province, south-west China, and characterized by using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain YIM 65188 is a member of the genus and exhibited 97.5 and 96.3 % gene sequence similarities to GW41-1564 and YIM 47672, respectively, whereas low sequence similarity values (<95.2 %) distinguished strain YIM 65188 from all other species with validly published names. Strain YIM 65188 had a unique menaquinone composition, with the predominant quinones being MK-11(H) and MK-10(H), with moderate amounts of MK-10(H), MK-11(H), MK-9(H), MK-11(H), MK-9(H) and MK-10(H). Similarly, the closest phylogenetic relative, GW41-1564, also had an unusual quinone composition, with the predominant menaquinones MK-10(H) and MK-10(H) and minor amounts of MK-9(H) and MK-9(H). The DNA–DNA hybridization value between strain YIM 65188 and GW41-1564 was 42.7 %. On the basis of phenotypic and phylogenetic characteristics, strain YIM 65188 was identified as a novel species of the genus for which the name sp. nov. is proposed, with YIM 65188 (=CCTCC AA 208020 =DSM 41942) as the type strain.


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