%0 Journal Article %A Aguilera, Margarita %A Jiménez-Pranteda, Maria L. %A Kharroub, Karima %A González-Paredes, Ana %A Durban, Juan J. %A Russell, Nick J. %A Ramos-Cormenzana, Alberto %A Monteoliva-Sánchez, Mercedes %T Marinobacter lacisalsi sp. nov., a moderately halophilic bacterium isolated from the saline-wetland wildfowl reserve Fuente de Piedra in southern Spain %D 2009 %J International Journal of Systematic and Evolutionary Microbiology, %V 59 %N 7 %P 1691-1695 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.007518-0 %I Microbiology Society, %X A Gram-negative, non-spore-forming, motile, moderately halophilic, aerobic, rod-shaped bacterium, designated strain FP2.5T, was isolated from the inland hypersaline lake Fuente de Piedra, a saline-wetland wildfowl reserve located in the province of Málaga in southern Spain. Strain FP2.5T was subjected to a polyphasic taxonomic study. It produced colonies with a light-yellow pigment. Strain FP2.5T grew at salinities of 3–15 % (w/v) and at temperatures of 20–40 °C. The pH range for growth was 5–9. Strain FP2.5T was able to utilize various organic acids as sole carbon and energy source. Its major fatty acids were C16 : 0, C18 : 1 ω9c and C16 : 1 ω9c. The DNA G+C content was 58.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FP2.5T appeared to be a member of the genus Marinobacter and clustered closely with the type strains of Marinobacter segnicrescens, Marinobacter bryozoorum and Marinobacter gudaonensis (levels of 16S rRNA gene sequence similarity of 98.1, 97.4 and 97.2 %, respectively). However, DNA–DNA relatedness between the new isolate and the type strains of its closest related Marinobacter species was low; levels of DNA–DNA relatedness between strain FP2.5T and M. segnicrescens LMG 23928T, M. bryozoorum DSM 15401T and M. gudaonensis DSM 18066T were 36.3, 32.1 and 24.9 %, respectively. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain FP2.5T is considered to represent a novel species of the genus Marinobacter, for which the name Marinobacter lacisalsi sp. nov. is proposed. The type strain is FP2.5T (=CECT 7297T=LMG 24237T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.007518-0