A Gram-negative, non-spore-forming, motile, moderately halophilic, aerobic, rod-shaped bacterium, designated strain FP2.5, was isolated from the inland hypersaline lake Fuente de Piedra, a saline-wetland wildfowl reserve located in the province of Málaga in southern Spain. Strain FP2.5 was subjected to a polyphasic taxonomic study. It produced colonies with a light-yellow pigment. Strain FP2.5 grew at salinities of 3–15 % (w/v) and at temperatures of 20–40 °C. The pH range for growth was 5–9. Strain FP2.5 was able to utilize various organic acids as sole carbon and energy source. Its major fatty acids were C, C 9 and C 9. The DNA G+C content was 58.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FP2.5 appeared to be a member of the genus and clustered closely with the type strains of , and (levels of 16S rRNA gene sequence similarity of 98.1, 97.4 and 97.2 %, respectively). However, DNA–DNA relatedness between the new isolate and the type strains of its closest related species was low; levels of DNA–DNA relatedness between strain FP2.5 and LMG 23928, DSM 15401 and DSM 18066 were 36.3, 32.1 and 24.9 %, respectively. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain FP2.5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FP2.5 (=CECT 7297=LMG 24237).


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vol. , part 7, pp. 1691 - 1695

Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences. [PDF](19 KB)


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