strain NRRL 5646, isolated from a garden soil sample in Osceola, Iowa, USA, was initially of interest as an antibiotic producer. It contained biocatalytically important enzymes and represented the first described nitric oxide synthase enzyme system in bacteria. The present polyphasic taxonomic study was undertaken to differentiate strain NRRL 5646 from related species of the genus . Chemotaxonomic analyses included determinations of the fatty acid methyl ester profile (C 6/C 7, C, C 9 and C 10-methyl as major components), quinone [cyclo MK-8(H) as the major component], polar lipid (diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside as major components) and mycolic acid. These results supported its placement within the genus . Biochemical testing and 16S rRNA, 65-kDa heat-shock protein () and preprotein translocase () gene sequence analyses differentiated strain NRRL 5646 from recognized species. Previous studies have demonstrated that other genetic sequences (carboxylic acid reductase, phosphopantetheinyl transferase and GTP cyclohydrolase I) from strain NRRL 5646 can also be used to substantiate its uniqueness. The level of 16S rRNA gene sequence similarity between strain NRRL 5646 and the type strains of and was 98.8 %. However, strain NRRL 5646 could be clearly distinguished from these species based on DNA–DNA hybridization data. Consequently, strain NRRL 5646 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NRRL 5646 (=UI 122540=NRRL B-24671=DSM 45197).


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