Three rhizobial strains (CCBAU 01393, CCBAU 01389 and CCBAU 03239) isolated from nodules of grown in saline-alkaline soils in the north of China had identical 16S rRNA genes that showed 99.7 and 99.5 % sequence similarities with those of SO2 and USDA 4128, respectively. Phylogenies of the housekeeping genes , and confirmed their distinct position, differing from recognized species. SDS-PAGE of whole-cell soluble proteins and a series of phenotypic and physiological tests allowed us to differentiate the novel group from all closely related recognized species. The levels of DNA–DNA relatedness between strain CCBAU 01393 and SO2 and USDA 4128 were 34.9 and 20.5 %, respectively. Therefore, we propose that strains CCBAU 01393, CCBAU 01389 and CCBAU 03239 represent a novel species, sp. nov., with strain CCBAU 01393 (=LMG 24763=HAMBI 3051) as the type strain. This strain could form effective nodules on , and .


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Phenogram derived from UPGMA/SSM analysis and SDS-PAGE analysis of whole-cell soluble proteins of strains CCBAU 01393 , CCBAU 01389 and CCBAU 03239. [PDF](95 KB)


Phylogenetic trees based on and genes. [PDF](20 KB)

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