1887

Abstract

A Gram-negative, aerobic, chemo-organotrophic, catalase- and oxidase-positive strain, designated AIMA4, was isolated from a chironomid (Diptera; Chironomidae) egg mass which was sampled from a waste stabilization pond in northern Israel. Phylogenetic analysis based on the 16S rRNA gene placed strain AIMA4 in the genus (class ). Strain AIMA4 shared 16S rRNA gene sequence similarity values of 96.9 % with JCM 9216 and <95.7 % with the type strains of other genera. Strain AIMA4 was found to be a non-motile coccobacillus or rod that did not grow under anaerobic conditions and did not produce acid from carbohydrates. Growth was observed at salinities from 0 to 2.5 % (w/v) NaCl, at pH values from 5.0 to 9.0 and at temperatures from 18 to 37 °C. The major fatty acids (>10 %) of strain AIMA4 were C 7, C and C 7. Phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine were the predominant components of the polar lipids. The major ubiquinone was Q-8. On the basis of phenotypic properties and phylogenetic distinctiveness, strain AIMA4 (=LMG 24400=DSM 19884) was classified in the genus as the type strain of a novel species for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007211-0
2009-12-01
2020-12-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/12/3025.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007211-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Broza, M. & Halpern, M. ( 2001; ). Pathogen reservoirs: Chironomid egg masses and Vibrio cholerae. Nature 412, 40
    [Google Scholar]
  3. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  4. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  5. Collins, M. D. & Jones, D. ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef]
    [Google Scholar]
  6. Felske, A., Rheims, H., Wolterink, A., Stackebrandt, E. & Akkermans, A. D. ( 1997; ). Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils. Microbiology 143, 2983–2989.[CrossRef]
    [Google Scholar]
  7. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  8. Halpern, M., Gancz, H., Broza, M. & Kashi, Y. ( 2003; ). Vibrio cholerae hemagglutinin/protease degrades chironomid egg masses. Appl Environ Microbiol 69, 4200–4204.[CrossRef]
    [Google Scholar]
  9. Halpern, M., Broza, Y. B., Mittler, S., Arakawa, E. & Broza, M. ( 2004; ). Chironomid egg masses as a natural reservoir of Vibrio cholerae non-O1 and non-O139 in freshwater habitats. Microb Ecol 47, 341–349.
    [Google Scholar]
  10. Halpern, M., Raats, D., Lavion, R. & Mittler, S. ( 2006; ). Dependent population dynamics between Chironomids (non-biting midges) and Vibrio cholerae. FEMS Microbiol Ecol 55, 98–104.[CrossRef]
    [Google Scholar]
  11. Halpern, M., Landsberg, O., Raats, D. & Rosenberg, E. ( 2007; ). Culturable and VBNC Vibrio cholerae; Interactions with chironomid egg masses and their bacterial population. Microb Ecol 53, 285–293.[CrossRef]
    [Google Scholar]
  12. Hiraishi, A. & Hoshino, Y. ( 1984; ). Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 30, 435–448.[CrossRef]
    [Google Scholar]
  13. Hiraishi, A. & Komagata, K. ( 1989; ). Effects of the growth medium composition on menaquinone homolog formation in Micrococcus luteus. J Gen Appl Microbiol 35, 311–318.[CrossRef]
    [Google Scholar]
  14. Hiraishi, A., Shin, Y. K. & Sugiyama, J. ( 1995a; ). Brachymonas denitrificans gen. nov., sp. nov., an aerobic chemoorganotrophic bacterium which contains rhodoquinones, and evolutionary relationships of rhodoquinone producers to bacterial species with various quinone classes. J Gen Appl Microbiol 41, 99–117.[CrossRef]
    [Google Scholar]
  15. Hiraishi, A., Shin, Y. K. & Sugiyama, J. ( 1995b; ). Brachymonas denitrificans gen. nov., sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 55. Int J Syst Bacteriol 45, 879–880.[CrossRef]
    [Google Scholar]
  16. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  18. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef]
    [Google Scholar]
  19. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef]
    [Google Scholar]
  20. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  21. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  22. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007211-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007211-0
Loading

Data & Media loading...

Supplements

Combined Figures [ PDF]

PDF

Cellular fatty acid contents of strain AIMA4 and DSM 15123 . [ PDF] 57 KB

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error