1887

Abstract

A Gram-positive, rod-shaped, strictly aerobic, spore-forming, moderately halophilic bacterium, designated strain SSK2-2, was isolated from fermented shrimp paste (ka-pi) produced in Thailand. It contained MK-7 as the predominant menaquinone and -diaminopimelic acid in the cell-wall peptidoglycan. The isolate grew at 8–43 °C, pH 6–9 and in 0.5–24 % (w/v) NaCl (optimum, 6–14 % NaCl). The major cellular fatty acids were anteiso-C and anteiso-C. Phosphatidylglycerol and diphosphatidylglycerol were the major polar lipid components. The DNA G+C content was 39.7 mol%. Comparative 16S rRNA gene sequence analyses showed that strain SSK2-2 was most closely related to KCTC 3821 with 98.7 % sequence similarity. Based on phenotypic and molecular features combined with DNA–DNA hybridization results (≤24.9 % with KCTC 3821), this strain represents a novel species of the genus for which the name sp. nov. is proposed; the type strain is SSK2-2 (=KCTC 13177=PCU 300=TISTR 1858).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007161-0
2009-09-01
2019-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2254.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007161-0&mimeType=html&fmt=ahah

References

  1. Albert, R. A., Archambault, J., Rosselló-Mora, R., Tindall, B. J. & Matheny, M. ( 2005; ). Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55, 2125–2130.[CrossRef]
    [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Forbes, L. ( 1981; ). Rapid flagella stain. J Clin Microbiol 13, 807–809.
    [Google Scholar]
  6. García, M. T., Gallego, V., Ventosa, A. & Mellado, E. ( 2005; ). Thalassobacillus devorans gen. nov., sp. nov., a moderately halophilic, phenol-degrading, Gram-positive bacterium. Int J Syst Evol Microbiol 55, 1789–1795.[CrossRef]
    [Google Scholar]
  7. Hao, M. V., Kocur, M. & Komagata, K. ( 1984; ). Marinococcus gen. nov., a new genus for motile cocci with meso-diaminopimelic acid in the cell wall; and Marinococcus albus sp. nov. and Marinococcus halophilus (Novitsky and Kushner) comb. nov. J Gen Appl Microbiol 30, 449–459.[CrossRef]
    [Google Scholar]
  8. Heyndrickx, M., Lebbe, L., Kersters, K., De Vos, P., Forsyth, G. & Logan, N. A. ( 1998; ). Virgibacillus: a new genus to accommodate Bacillus panthothenticus (Proom and Knight 1950). Emended description of Virgibacillus panthothenticus. Int J Syst Bacteriol 48, 99–106.[CrossRef]
    [Google Scholar]
  9. Heyrman, J., Logan, N. A., Busse, H.-J., Balcaen, A., Lebbe, L., Rodriguez-Diaz, M., Swings, J. & De Vos, P. ( 2003; ). Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol 53, 501–511.[CrossRef]
    [Google Scholar]
  10. Hucker, G. J. & Conn, H. J. ( 1923; ). Method of Gram staining. N Y State Agric Exp Stn Tech Bull 93, 3–37.
    [Google Scholar]
  11. Ishikawa, M., Ishizaki, S., Yamamoto, Y. & Yamasato, K. ( 2002; ). Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga. J Gen Appl Microbiol 48, 269–279.[CrossRef]
    [Google Scholar]
  12. Ishikawa, M., Nakajima, K., Itamiya, Y., Furukawa, S., Yamamoto, Y. & Yamasato, K. ( 2005; ). Halolactibacillus halophilus gen. nov., sp. nov. and Halolactibacillus miurensis sp. nov., halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in Bacillus rRNA group 1. Int J Syst Evol Microbiol 55, 2427–2439.[CrossRef]
    [Google Scholar]
  13. Jeon, C. O., Lim, J.-M., Lee, J.-M., Xu, L.-H., Jiang, C.-L. & Kim, C.-J. ( 2005; ). Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55, 1891–1896.[CrossRef]
    [Google Scholar]
  14. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  15. Kim, Y. G., Choi, D. H., Hyun, S. & Cho, B. C. ( 2007; ). Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. Int J Syst Evol Microbiol 57, 409–413.[CrossRef]
    [Google Scholar]
  16. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  17. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  18. Lee, J.-S., Lim, J.-M., Lee, K. C., Lee, J.-C., Park, Y.-H. & Kim, C.-J. ( 2006; ). Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol 56, 251–257.[CrossRef]
    [Google Scholar]
  19. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  20. Lim, J.-M., Jeon, C. O., Song, S. M. & Kim, C.-J. ( 2005; ). Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55, 165–170.[CrossRef]
    [Google Scholar]
  21. Lu, J., Nogi, Y. & Takami, H. ( 2001; ). Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205, 291–297.[CrossRef]
    [Google Scholar]
  22. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  23. Nakamura, K., Haruta, S., Ueno, S., Ishii, M., Yokota, A. & Igarashi, Y. ( 2004; ). Cerasibacillus quisquiliarum gen. nov., sp. nov., isolated from a semi-continuous decomposing system of kitchen refuse. Int J Syst Evol Microbiol 54, 1063–1069.[CrossRef]
    [Google Scholar]
  24. Nam, J.-H., Bae, W. & Lee, D.-H. ( 2008; ). Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea. Int J Syst Evol Microbiol 58, 1109–1113.[CrossRef]
    [Google Scholar]
  25. Namwong, S., Tanasupawat, S., Smitinont, T., Visessanguan, W., Kudo, T. & Itoh, T. ( 2005; ). Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. from fish sauce in Thailand. Int J Syst Evol Microbiol 55, 315–320.[CrossRef]
    [Google Scholar]
  26. Niimura, Y., Koh, E., Yanagida, F., Suzuki, K.-I., Komagata, K. & Kozaki, M. ( 1990; ). Amphibacillus xylanus gen. nov., sp. nov., a facultatively anaerobic sporeforming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase. Int J Syst Bacteriol 40, 297–301.[CrossRef]
    [Google Scholar]
  27. Phithakpol, B., Varanyanond, W., Reungmaneepaitoon, S. & Wood, H. ( 1995; ). The Traditional Fermented Foods of Thailand. Kuala Lumpur: ASEAN Food Handling Bureau Level 3.
  28. Raats, D. & Halpern, M. ( 2007; ). Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. Int J Syst Evol Microbiol 57, 255–259.[CrossRef]
    [Google Scholar]
  29. Ren, P.-G. & Zhou, P.-J. ( 2005a; ). Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 95–99.[CrossRef]
    [Google Scholar]
  30. Ren, P.-G. & Zhou, P.-J. ( 2005b; ). Salinibacillus aidingensis gen. nov., sp. nov. and Salinibacillus kushneri sp. nov., moderately halophilic bacteria isolated from a neutral saline lake in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 949–953.[CrossRef]
    [Google Scholar]
  31. Romano, I., Lama, L., Nicolaus, B., Poli, A., Gambacorta, A. & Giordano, A. ( 2006; ). Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi. Int J Syst Evol Microbiol 56, 805–810.[CrossRef]
    [Google Scholar]
  32. Saito, H. & Miura, K. I. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  33. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  34. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  35. Satomi, M., Kimura, B., Mizoi, M., Sato, T. & Fujii, T. ( 1997; ). Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. Int J Syst Bacteriol 47, 832–836.[CrossRef]
    [Google Scholar]
  36. Schlesner, H., Lawson, P. A., Collins, M. D., Weiss, N., Wehmeyer, U., Völker, H. & Thomm, M. ( 2001; ). Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51, 425–431.
    [Google Scholar]
  37. Seearunruangchai, A., Tanasupawat, S., Keeratipibul, S., Thawai, C., Itoh, T. & Yamada, Y. ( 2004; ). Identification of acetic acid bacteria isolated from fruits collected in Thailand. J Gen Appl Microbiol 50, 47–53.[CrossRef]
    [Google Scholar]
  38. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K.-H. ( 1996; ). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46, 492–496.[CrossRef]
    [Google Scholar]
  39. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  40. Tanasupawat, S. & Komagata, K. ( 2001; ). Lactic acid bacteria in fermented foods in Southeast Asia. In Microbial Diversity in Asia. Technology and Prospects, pp. 43–59. Edited by B. H. Nga, H. M. Tan & K. Suzuki. Singapore: World Scientific Publishing.
  41. Tanasupawat, S., Shida, O., Okada, S. & Komagata, K. ( 2000; ). Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50, 1479–1485.[CrossRef]
    [Google Scholar]
  42. Tanasupawat, S., Namwong, S., Kudo, T. & Itoh, T. ( 2007; ). Piscibacillus salipiscarius gen. nov., sp. nov., a moderately halophilic bacterium from fermented fish (pla-ra) in Thailand. Int J Syst Evol Microbiol 57, 1413–1417.[CrossRef]
    [Google Scholar]
  43. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  44. Thornley, M. J. ( 1960; ). The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. J Appl Bacteriol 23, 37–52.[CrossRef]
    [Google Scholar]
  45. Valderrama, M. J., Monteoliva-Sanchez, M., Quesada, E. & Ramos-Cormenzana, A. ( 1998; ). Influence of salt concentration on the cellular fatty acid composition of the moderately halophilic bacterium Halomonas salina. Res Microbiol 149, 675–679.[CrossRef]
    [Google Scholar]
  46. Ventosa, A., Garcia, M. T., Kamekura, M., Onishi, H. & Ruiz-Berraquero, F. ( 1989; ). Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Syst Appl Microbiol 12, 162–166.[CrossRef]
    [Google Scholar]
  47. Wainø, M., Tindall, B. J., Schumann, P. & Ingvorsen, K. ( 1999; ). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49, 821–831.[CrossRef]
    [Google Scholar]
  48. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  49. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2002; ). Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 52, 2043–2048.[CrossRef]
    [Google Scholar]
  50. Yumoto, I., Hirota, K., Nodasaka, Y. & Nakajima, K. ( 2005; ). Oceanobacillus oncorhynchi sp. nov., a halotolerant obligate alkaliphile isolated from the skin of a rainbow trout (Oncorhynchus mykiss), and emended description of the genus Oceanobacillus. Int J Syst Evol Microbiol 55, 1521–1524.[CrossRef]
    [Google Scholar]
  51. Zhilina, T. N., Garnova, E. S., Tourova, T. P., Kostrikina, N. A. & Zavarzin, G. A. ( 2001; ). Amphibacillus fermentum sp. nov. and Amphibacillus tropicus sp. nov., new alkaliphilic, facultatively anaerobic, saccharolytic bacilli from Lake Magadi. Microbiology 70, 711–722. (English translation of Mikrobiologiia)[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007161-0
Loading
/content/journal/ijsem/10.1099/ijs.0.007161-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 2254 - 2259

Cellular fatty acid compositions of strain SSK2-2 and related taxa. [PDF](45 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error