A novel aerobic, obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium, S10, was isolated from hot-spring sediment samples collected from Atri, Bhubaneswar, India. The cells of this isolate stained Gram-negative and were strictly aerobic, non-sporulating, rod-shaped and motile with a single polar flagellum. Strain S10 was positive for oxidase and catalase activities. It was capable of utilizing thiosulfate under mixotrophic growth conditions. Mixotrophic growth was observed at pH 6.0–8.5 and 25–45 °C; optimum growth occurred at pH 7.5–8.0 and 30–37 °C. The major cellular fatty acids were C 3-OH, C 7, C, C cyclo, C 7 and C cyclo 8. The DNA G+C content of strain S10 was 64.8 mol%. 16S rRNA gene sequence analysis indicated that the bacterium clustered within the radiation of the genus and showed 98.0 % similarity with ATCC 23370 and ATCC 15466. However, DNA–DNA reassociation values of strain S10 with JCM 20426 and JCM 20425, its nearest phylogenetic relatives, were 46 and 39 %, respectively. On the basis of phenotypic, physiological and chemotaxonomic properties, 16S rRNA gene sequence analysis and DNA–DNA reassociation studies, it is proposed that strain S10 represents a novel species of the genus , sp. nov.; the type strain is S10 (=DSM 18181 =JCM 14806).


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