1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 9

sp. nov., an obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium Free

Abstract

A novel aerobic, obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium, S10, was isolated from hot-spring sediment samples collected from Atri, Bhubaneswar, India. The cells of this isolate stained Gram-negative and were strictly aerobic, non-sporulating, rod-shaped and motile with a single polar flagellum. Strain S10 was positive for oxidase and catalase activities. It was capable of utilizing thiosulfate under mixotrophic growth conditions. Mixotrophic growth was observed at pH 6.0–8.5 and 25–45 °C; optimum growth occurred at pH 7.5–8.0 and 30–37 °C. The major cellular fatty acids were C 3-OH, C 7, C, C cyclo, C 7 and C cyclo 8. The DNA G+C content of strain S10 was 64.8 mol%. 16S rRNA gene sequence analysis indicated that the bacterium clustered within the radiation of the genus and showed 98.0 % similarity with ATCC 23370 and ATCC 15466. However, DNA–DNA reassociation values of strain S10 with JCM 20426 and JCM 20425, its nearest phylogenetic relatives, were 46 and 39 %, respectively. On the basis of phenotypic, physiological and chemotaxonomic properties, 16S rRNA gene sequence analysis and DNA–DNA reassociation studies, it is proposed that strain S10 represents a novel species of the genus , sp. nov.; the type strain is S10 (=DSM 18181 =JCM 14806).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.007120-0
2009-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2171.html?itemId=/content/journal/ijsem/10.1099/ijs.0.007120-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  2. Bhadra, B., Raghukumar, C., Pindi, P. K. & Shivaji, S.(2008).Brevibacterium oceani sp. nov., isolated from deep-sea sediment of the Chagos Trench, Indian Ocean. Int J Syst Evol Microbiol 58, 57–60.[CrossRef] [Google Scholar]
  3. Cruickshank, R., Duguid, J. P., Marmion, B. P. & Swain, R. H. A. (editors)(1975). In Medical Microbiology, 12th edn, vol. 2, The Practice of Medical Microbiology, pp. 301–310. Edinburgh: Churchill Livingstone.
  4. Das, S. K., Mishra, A. K., Tindall, B. J., Rainey, F. A. & Stackebrandt, E.(1996). Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (strain BI-42) gen. nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing. Int J Syst Bacteriol 46, 981–987.[CrossRef] [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Ghosh, D., Bal, B., Kashyap, V. K. & Pal, S.(2003). Molecular phylogenetic exploration of bacterial diversity in a Bakreshwar (India) hot spring and culture of Shewanella-related thermophiles. Appl Environ Microbiol 69, 4332–4336.[CrossRef] [Google Scholar]
  8. Gupta, M. L., Narain, H. & Saxena, V. K.(1975). Geochemistry of thermal waters from various geothermal provinces of India. In Proceedings of the Grenoble Symposium of the International Association of Hydrological Sciences, pp. 47–58. Wallingford, UK: IAHS Press.
  9. Huber, H. & Stetter, K. O.(1990).Thiobacillus cuprinus sp. nov., a novel facultatively organotrophic metal-mobilizing bacterium. Appl Environ Microbiol 56, 315–322. [Google Scholar]
  10. Katayama, Y., Uchino, Y., Wood, A. P. & Kelly, D. P.(2006). Confirmation of Thiomonas delicata (formerly Thiobacillus delicatus) as a distinct species of the genus Thiomonas Moreira and Amils 1997 with comments on some species currently assigned to the genus. Int J Syst Evol Microbiol 56, 2553–2557.[CrossRef] [Google Scholar]
  11. Katayama-Fujimura, Y., Tsuzaki, N. & Kuraishi, H.(1982). Ubiquinone, fatty acid and DNA base composition determination as a guide to the taxonomy of the genus Thiobacillus. J Gen Microbiol 128, 1599–1611. [Google Scholar]
  12. Katayama-Fujimura, Y., Kawashima, I., Tsuzaki, N. & Kuraishi, H.(1984). Physiological characteristics of the facultatively chemolithotrophic Thiobacillus species Thiobacillus delicatus nom. rev., emend., Thiobacillus perometabolis, and Thiobacillus intermedius. Int J Syst Bacteriol 34, 139–144.[CrossRef] [Google Scholar]
  13. Kelly, D. P. & Wood, A. P.(2005). Genus incertae sedis XVIII. Thiomonas Moreira and Amils 1997, 527VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 757–759. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  14. Kelly, D. P., Wood, A. P. & Stackebrandt, E.(2005). Genus II. Thiobacillus Beijerinck 1904b, 597AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 764–769. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  15. Kelly, D. P., Uchino, Y., Huber, H., Amils, R. & Wood, A. P.(2007). Reassessment of the phylogenetic relationships of Thiomonas cuprina. Int J Syst Evol Microbiol 57, 2720–2724.[CrossRef] [Google Scholar]
  16. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  17. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  18. Kuykendall, L. D., Roy, M. D., O'Neill, J. J. & Devine, T. E.(1988). Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef] [Google Scholar]
  19. Maidak, B. L., Olsen, G. J., Larsen, N., Overbeek, R., McCaughey, M. J. & Woese, C. R.(1997). The RDP (Ribosomal Database Project). Nucleic Acids Res 25, 109–111.[CrossRef] [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  21. Moreira, D. & Amils, R.(1997). Phylogeny of Thiobacillus cuprinus and other mixotrophic thiobacilli: proposal for Thiomonas gen. nov. Int J Syst Bacteriol 47, 522–528.[CrossRef] [Google Scholar]
  22. Moreira, C., Rainey, F. A., Nobre, M. F., da Silva, M. T. & da Costa, M. S.(2000).Tepidimonas ignava gen. nov., sp. nov., a new chemolithoheterotrophic and slightly thermophilic member of the β-Proteobacteria. Int J Syst Evol Microbiol 50, 735–742.[CrossRef] [Google Scholar]
  23. Odintsova, E. V., Jannasch, H. W., Mamone, J. A. & Langworthy, T. A.(1996).Thermothrix azorensis sp. nov., an obligately chemolithoautotrophic, sulfur-oxidizing, thermophilic bacterium. Int J Syst Bacteriol 46, 422–428.[CrossRef] [Google Scholar]
  24. Patra, M., Pandey, S. K., Ramamurthy, T., Singh, D. V. & Das, S. K.(2007). Characterization of cytotoxin-producing Aeromonas caviae (strain HT10) isolated from a sulfur spring in Orissa, India. Lett Appl Microbiol 44, 338–341.[CrossRef] [Google Scholar]
  25. Sharma, D. P., Thomas, C., Hall, R. H., Levine, M. M. & Attridge, S. R.(1989). Significance of toxin coregulated pili as protective antigens of Vibrio cholerae in the infant mouse model. Vaccine 7, 451–456.[CrossRef] [Google Scholar]
  26. Shooner, F., Bousquet, J. & Tyagi, R. D.(1996). Isolation, phenotypic characterization, and phylogenetic position of a novel, facultatively autotrophic, moderately thermophilic bacterium, Thiobacillus thermosulfatus sp. nov. Int J Syst Bacteriol 46, 409–415.[CrossRef] [Google Scholar]
  27. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  28. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  30. Wood, A. P. & Kelly, D. P.(1988). Isolation and physiological characterisation of Thiobacillus aquaesulis sp. nov., a novel facultatively autotrophic moderate thermophile. Arch Microbiol 149, 339–343.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.007120-0
Loading
Thiomonas bhubaneswarensis sp. nov., an obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium
Int J Syst Evol Microbiol 59, 2171 (2009); https://doi.org/10.1099/ijs.0.007120-0
/content/journal/ijsem/10.1099/ijs.0.007120-0
/content/journal/ijsem/10.1099/ijs.0.007120-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error