gen. nov., sp. nov., a new member of the -2 subgroup of the Free

Abstract

A Gram-negative, rod-shaped, facultatively anaerobic, halotolerant bacterial strain, designated YIM YD3, was isolated from a salt mine in Yunnan, south-west China. The taxonomy of strain YIM YD3 was investigated by a polyphasic approach. Strain YIM YD3 was motile, formed pink colonies and was positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and two unknown phospholipids. The major fatty acids (>10 % of total fatty acids) were C 7, C 9, C and C cyclo 8. The DNA G+C content was 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate formed a distinct line within a clade containing the genera , , and in the order , with highest levels of 16S RNA gene sequence similarity to the type strain of (93.5 %). On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain YIM YD3 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed, with strain YIM YD3 (=KCTC 22346=CCTCC AA208038) as the type strain.

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2010-01-01
2024-03-28
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References

  1. Allgaier M., Uphoff H., Felske A., Wagner-Döbler I. 2003; Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. Appl Environ Microbiol 69:5051–5059 [CrossRef]
    [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  3. Auling G., Busse H.-J., Egli T., El-Banna T., Stackebrandt E. 1993 Description of the gram-negative, obligately aerobic, nitrilotriacetate (NTA)-utilizing bacteria as Chelatobacter heintzii , gen.nov., sp. nov., and Chelatococcus asaccharovorans , gen. nov., sp. nov. Syst Appl Microbiol 16104–112
  4. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496
    [Google Scholar]
  5. Cho J. C., Giovannoni S. J. 2006; Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 56:855–859 [CrossRef]
    [Google Scholar]
  6. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [CrossRef]
    [Google Scholar]
  7. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [CrossRef]
    [Google Scholar]
  8. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  9. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  10. Cui X. L., Mao P. H., Zeng M., Li W. J., Zhang L. P., Xu L. H., Jiang C. L. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363
    [Google Scholar]
  11. Das S. K., Mishra A. K., Tindall B. J., Rainey F. A., Stackebrandt E. 1996; Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (strain BI-42) gen. nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing. Int J Syst Bacteriol 46:981–987 [CrossRef]
    [Google Scholar]
  12. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  13. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  14. Garrity G. M., Bell J. A., Lilburn T. G. 2004; Taxonomic outline of the prokaryotes. In Bergey's Manual of Systematic Bacteriology , 2nd edn, release 5.0. New York: Springer; http://www.bergeys.org/outlines/bergeysoutline_5_2004.pdf
    [Google Scholar]
  15. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [CrossRef]
    [Google Scholar]
  16. Hwang C. Y., Cho B. C. 2008; Cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order Rhizobiales , and proposal of Cohaesibacteraceae fam. nov. Int J Syst Evol Microbiol 58:267–277 [CrossRef]
    [Google Scholar]
  17. Kanso S. W., Patel B. K. C. 2003; Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer. Int J Syst Evol Microbiol 53:401–406 [CrossRef]
    [Google Scholar]
  18. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  19. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by oxidase reaction. Nature 178: 703
    [Google Scholar]
  20. La Scola B., Mallet M. N., Grimont P. A. D., Raoult D. 2003; Bodea eneae sp. nov., Bosea massiliensis sp.nov. and Bosea vestrisii sp. nov.,isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996). Int J Syst Evol Microbiol 53:15–20 [CrossRef]
    [Google Scholar]
  21. Lee K. B., Liu C. T., Anzai Y., Kim H., Aono T., Oyaizu H. 2005; The hierarchical system of the ‘ Alphaproteobacteria ’: description of Hyphomonadaceae fam.nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55:1907–1919 [CrossRef]
    [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas , Oerskovia and related taxa. J Appl Microbiol 47:87–95
    [Google Scholar]
  24. Ouattara A. S., Assih E. A., Thierry S., Cayol J. L., Labat M., Monroy O., Macarie H. 2003; Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester. Int J Syst Evol Microbiol 53:1247–1251 [CrossRef]
    [Google Scholar]
  25. Qu J. H., Yuan H. L., Wang E. T., Li C., Huang H. Z. 2008; Bacterial diversity in sediments of the eutrophic Guanting Reservoir, China, estimated by analyses of 16S rDNA sequence. Biodivers Conserv 17:1667–1683 [CrossRef]
    [Google Scholar]
  26. Quesada E., Béjar V., Calvo C. 1993; Exopolysaccharide production by Volcaniella eurihalina . Experientia 49:1037–1041 [CrossRef]
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  28. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20: 16
    [Google Scholar]
  29. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  30. Takeda M., Suzuki I., Koizumi J. 2004; Balneomonas flocculans gen. nov., sp. nov. a new cellulose-producing member of the α -2 subclass of Proteobacteria . Syst Appl Microbiol 27139–145 [CrossRef]
    [Google Scholar]
  31. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Microbiol 54:31–36
    [Google Scholar]
  32. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  33. Woese C. R., Stackebrandt E., Weisburg W. G., Paster B. J., Madigan M. T., Fowler V. J., Hahn C. M., Blanz P., Gupta R. other authors 1984; The phylogeny of purple bacteria: the alpha subdivision. Syst Appl Microbiol 5:315–326 [CrossRef]
    [Google Scholar]
  34. Yoon J.-H., Kang S.-J., Im W.-T., Lee S.-T., Oh T.-K. 2008; Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus . Int J Syst Evol Microbiol 58:2224–2228 [CrossRef]
    [Google Scholar]
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