A Gram-negative, rod-shaped, facultatively anaerobic, halotolerant bacterial strain, designated YIM YD3T, was isolated from a salt mine in Yunnan, south-west China. The taxonomy of strain YIM YD3T was investigated by a polyphasic approach. Strain YIM YD3T was motile, formed pink colonies and was positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and two unknown phospholipids. The major fatty acids (>10 % of total fatty acids) were C18 : 1ω7c, C18 : 1ω9c, C16 : 0 and C19 : 0 cyclo ω8c. The DNA G+C content was 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate formed a distinct line within a clade containing the genera Balneimonas, Bosea, Chelatococcus and Microvirga in the order Rhizobiales, with highest levels of 16S RNA gene sequence similarity to the type strain of Balneimonas flocculans (93.5 %). On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain YIM YD3T represents a novel species in a new genus, for which the name Salinarimonas rosea gen. nov., sp. nov. is proposed, with strain YIM YD3T (=KCTC 22346T=CCTCC AA208038T) as the type strain.
AllgaierM.,
UphoffH.,
FelskeA.,
Wagner-DöblerI.2003; Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. Appl Environ Microbiol 69:5051–5059[CrossRef]
AulingG.,
BusseH.-J.,
EgliT.,
El-BannaT.,
StackebrandtE.1993 Description of the gram-negative, obligately aerobic, nitrilotriacetate (NTA)-utilizing bacteria as Chelatobacter heintzii , gen.nov., sp. nov., and Chelatococcus asaccharovorans , gen. nov., sp. nov. Syst Appl Microbiol 16104–112
BauerA. W.,
KirbyW. M. M.,
SherrisJ. C.,
TurckM.1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496
ChoJ. C.,
GiovannoniS. J.2006; Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales
. Int J Syst Evol Microbiol 56:855–859[CrossRef]
CollinsM. D.,
JonesD.1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470[CrossRef]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[CrossRef]
CuiX. L.,
MaoP. H.,
ZengM.,
LiW. J.,
ZhangL. P.,
XuL. H.,
JiangC. L.2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae
. Int J Syst Evol Microbiol 51:357–363
DasS. K.,
MishraA. K.,
TindallB. J.,
RaineyF. A.,
StackebrandtE.1996; Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (strain BI-42) gen. nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing. Int J Syst Bacteriol 46:981–987[CrossRef]
GarrityG. M.,
BellJ. A.,
LilburnT. G.2004; Taxonomic outline of the prokaryotes. In Bergey's Manual of Systematic Bacteriology , 2nd edn, release 5.0. New York: Springer; http://www.bergeys.org/outlines/bergeysoutline_5_2004.pdf
HwangC. Y.,
ChoB. C.2008; Cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order Rhizobiales , and proposal of Cohaesibacteraceae fam. nov. Int J Syst Evol Microbiol 58:267–277[CrossRef]
KansoS. W.,
PatelB. K. C.2003; Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer. Int J Syst Evol Microbiol 53:401–406[CrossRef]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[CrossRef]
La ScolaB.,
MalletM. N.,
GrimontP. A. D.,
RaoultD.2003; Bodea eneae sp. nov., Bosea massiliensis sp.nov. and Bosea vestrisii sp. nov.,isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996). Int J Syst Evol Microbiol 53:15–20[CrossRef]
LeeK. B.,
LiuC. T.,
AnzaiY.,
KimH.,
AonoT.,
OyaizuH.2005; The hierarchical system of the ‘ Alphaproteobacteria ’: description of Hyphomonadaceae fam.nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55:1907–1919[CrossRef]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
MinnikinD. E.,
CollinsM. D.,
GoodfellowM.1979; Fatty acid and polar lipid composition in the classification of Cellulomonas , Oerskovia and related taxa. J Appl Microbiol 47:87–95
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology
. pp 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
TakedaM.,
SuzukiI.,
KoizumiJ.2004; Balneomonas flocculans gen. nov., sp. nov. a new cellulose-producing member of the α -2 subclass of Proteobacteria
. Syst Appl Microbiol27139–145[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
YoonJ.-H.,
KangS.-J.,
ImW.-T.,
LeeS.-T.,
OhT.-K.2008; Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus
. Int J Syst Evol Microbiol 58:2224–2228[CrossRef]