A Gram-positive-staining, aerobic, catalase- and oxidase-positive, irregular short rod-shaped actinobacterium, designated strain YIM C003, was isolated from a salt mine in Yunnan, PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM C003 was most closely related to strains of the genera (94.2–96.0 % similarity), (95.6 %), (94.9–95.4 %), (94.9–95.4 %), (95.0 %), (95.0 %) and (94.2–95.0 %) of the family and that it formed an independent monophyletic lineage with three strains of . The DNA G+C content of strain YIM C003 was 72.0 mol%. The diagnostic cell-wall diamino acid was -diaminopimelic acid. The predominant menaquinone was MK-8(H). Mycolic acids were not detected. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids. The major cellular fatty acids were C 9 and C. These chemotaxonomic properties, together with data from phylogenetic analysis, enabled the novel isolate to be differentiated from all other members of the family. A novel species in a new genus, gen. nov., sp. nov., is proposed, with strain YIM C003 (=DSM 21208 =CCTCC AA 208036) as the type strain of .


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vol. , part 9, pp. 2185 - 2190

Transmission electron micrograph of cells of strain YIM C003 grown on GTM agar medium for 7 days at 28 °C. Bar, 1 µm.

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