A Gram-positive-staining, coccoid-shaped, oxidase-negative, non-spore-forming, non-motile bacterium, strain KSS-17Se, was isolated from a metalworking fluid. On the basis of its major fatty acid (ai-C) and 16S rRNA gene sequence similarity, the strain grouped with and , sharing 95.3 and 97.4 % 16S rRNA gene sequence similarity with the respective type strains. Similarities with other established species of the genera , and were lower than 95.5 %. The quinone system was characterized by the major menaquinone MK-9(H). In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid and an unknown polar lipid were detected as major compounds. Additionally, three unknown glycolipids and minor amounts of phosphatidylethanolamine, two unknown aminolipids and two unknown polar lipids were detected. Phosphatidylinositol was present only in trace amounts. Predominant polyamines were spermine and spermidine. -Diaminopimelic acid was identified as the diagnostic diamino acid in the cell wall. The strain showed clear differences in phenotype (including chemotaxonomic features) from both species and members of the other above-mentioned genera. DNA–DNA hybridization between KSS-17Se and Ben-106 and SST-39 yielded similarities of 15.1 and 21.0 %, respectively. It is evident that the organism represents a novel species, for which the name sp. nov. is proposed. The type strain is KSS-17Se (=DSM 19926 =CCUG 55516).


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vol. , part 6, pp. 1545 - 1549

Polar lipid profile of strain KSS-17Se after two-dimensional TLC and detection with molybdatophosphoric acid. PG, Phosphatidylglycerol; DPG, diphosphatidylglycerol; PE, phosphatidyethanolamine; AL1, AL2, unknown aminolipids; L1–L3, unknown polar lipids; GL1–GL4, unknown glycolipids.


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