RT Journal Article SR Electronic(1) A1 Houf, Kurt A1 On, Stephen L. W. A1 Coenye, Tom A1 Debruyne, Lies A1 De Smet, Sarah A1 Vandamme, PeterYR 2009 T1 Arcobacter thereius sp. nov., isolated from pigs and ducks JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 10 SP 2599 OP 2604 DO https://doi.org/10.1099/ijs.0.006650-0 PB Microbiology Society, SN 1466-5034, AB During a Danish study on the prevalence of campylobacteria in pig abortions and food of animal origin, eight Gram-negative, slightly curved, rod-shaped, non-spore-forming bacteria were clustered by using amplified fragment length polymorphism analysis in a distinct phenon within the genus Arcobacter. In the present study, numerical analysis of whole-cell protein profiles also showed that all isolates clustered in a single group distinct from other recognized Arcobacter species. DNA–DNA hybridization among two representative strains exhibited a mean DNA–DNA relatedness value of 79 %. DNA–DNA hybridization with the type strains of recognized Arcobacter species revealed levels of DNA–DNA relatedness of 41 % or less. The DNA G+C content of the type strain was 28.5 mol%. Pairwise comparison of the 16S rRNA gene sequences with those of the type strains of established species identified Arcobacter cryaerophilus (97.9 %), Arcobacter cibarius (97.5 %) and Arcobacter skirrowii (97.2 %) as the nearest phylogenetic neighbours. The isolates could be distinguished from other Arcobacter species by means of the following biochemical tests: activities of catalase and urease, reduction of nitrate and growth on minimal medium, lack of growth at 37 °C under standardized aerobic and microaerobic conditions, in 4 % NaCl and 1 % glycine media. Finally, DNA fingerprints obtained by using enterobacterial repetitive intergenic consenus-PCR showed that the eight isolates represent eight strains of a single novel Arcobacter species, for which the name Arcobacter thereius sp. nov. is proposed. The type strain is LMG 24486T (=CCUG 56902T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.006650-0