1887

Abstract

During a Danish study on the prevalence of campylobacteria in pig abortions and food of animal origin, eight Gram-negative, slightly curved, rod-shaped, non-spore-forming bacteria were clustered by using amplified fragment length polymorphism analysis in a distinct phenon within the genus . In the present study, numerical analysis of whole-cell protein profiles also showed that all isolates clustered in a single group distinct from other recognized species. DNA–DNA hybridization among two representative strains exhibited a mean DNA–DNA relatedness value of 79 %. DNA–DNA hybridization with the type strains of recognized species revealed levels of DNA–DNA relatedness of 41 % or less. The DNA G+C content of the type strain was 28.5 mol%. Pairwise comparison of the 16S rRNA gene sequences with those of the type strains of established species identified (97.9 %), (97.5 %) and (97.2 %) as the nearest phylogenetic neighbours. The isolates could be distinguished from other species by means of the following biochemical tests: activities of catalase and urease, reduction of nitrate and growth on minimal medium, lack of growth at 37 °C under standardized aerobic and microaerobic conditions, in 4 % NaCl and 1 % glycine media. Finally, DNA fingerprints obtained by using enterobacterial repetitive intergenic consenus-PCR showed that the eight isolates represent eight strains of a single novel species, for which the name sp. nov. is proposed. The type strain is LMG 24486 (=CCUG 56902).

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2009-10-01
2022-01-18
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Dendrogram derived from numerical analysis of whole-cell protein profiles of sp. nov. and reference strains. [PDF](54 KB)

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DNA fingerprints obtained using enterobacterial repetitive intergenic consenus-PCR of sp. nov. and reference strains. [PDF](262 KB)

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