1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 7

gen. nov., sp. nov., a member of the family that exhibits swimming motility, isolated from desert soil Free

Abstract

A Gram-negative and rod-shaped bacterial strain, X-65, isolated from desert soil of Xinjiang, China, was subjected to phenotypic and phylogenetic studies. On the basis of 16S rRNA gene sequence analyses, the isolate showed closest relationship to members of the genera (92.4–95.4 % similarity), (94.0 %), (93.9 %) and (93.8 %) and to LMG 11607 (93.3 %). The strain differed from its phylogenetic neighbours by its swimming motility in liquid cultures and the presence of long appendages and large amounts of the straight-chain fatty acids 16 : 0 and 18 : 0. Growth was observed at 18–37 °C (optimum 30 °C), at pH 6–12 (optimum pH 7–8) and in the presence of 0–3 % NaCl (optimum 0 %). Flexirubin pigments were absent. The major isoprenoid quinone was MK-6. The DNA G+C content of X-65 was 41.5 mol%. The data presented in this study indicate that the isolate should be classified in a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is X-65 (=CCTCC AB 208154 =NRRL B-51322).

  • Published Online:
Keyword(s): CBR, Chryseobacterium–Bergeyella–Riemerella
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.006619-0
2009-07-01
2023-01-30
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/7/1672.html?itemId=/content/journal/ijsem/10.1099/ijs.0.006619-0&mimeType=html&fmt=ahah

References

  1. Allen, T. D., Lawson, P. A., Collins, M. D., Falsen, E. & Tanner, R. S.(2006).Cloacibacterium normanense gen. nov., sp. nov., a novel bacterium from the family Flavobacteriaceae isolated from municipal wastewater. Int J Syst Evol Microbiol 56, 1311–1316.[CrossRef] [Google Scholar]
  2. Bae, S. S., Kwon, K. K., Yang, S. H., Lee, H.-S., Kim, S.-J. & Lee, J.-H.(2007).Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome. Int J Syst Evol Microbiol 57, 1050–1054.[CrossRef] [Google Scholar]
  3. Bernardet, J.-F. & Nakagawa, Y.(2006). An introduction to the family Flavobacteriaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 455–480. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  4. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  5. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  6. Bernardet, J.-F., Hugo, C. & Bruun, B.(2006). The genera Chryseobacterium and Elizabethkingia. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 638–676. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  7. Bowman, J. P.(2000). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868. [Google Scholar]
  8. Bruns, A., Rohde, M. & Berthe-Corti, L.(2001).Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef] [Google Scholar]
  9. Buck, J. D.(1982). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  10. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  11. Gallego, V., Garcia, M. T. & Ventosa, A.(2006).Chryseobacterium hispanicum sp. nov., isolated from the drinking water distribution system of Sevilla, Spain. Int J Syst Evol Microbiol 56, 1589–1592.[CrossRef] [Google Scholar]
  12. Gosink, J. J., Woese, C. R. & Staley, J. T.(1998).Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48, 223–235.[CrossRef] [Google Scholar]
  13. Hantsis-Zacharov, E. & Halpern, M.(2007).Chryseobacterium haifense sp. nov., a psychrotolerant bacterium isolated from raw milk. Int J Syst Evol Microbiol 57, 2344–2348.[CrossRef] [Google Scholar]
  14. Herzog, P., Winkler, I., Wolking, D., Kämpfer, P. & Lipski, A.(2008).Chryseobacterium ureilyticum sp. nov., Chryseobacterium gambrini sp. nov., Chryseobacterium pallidum sp. nov. and Chryseobacterium molle sp. nov., isolated from beer-bottling plants. Int J Syst Evol Microbiol 58, 26–33.[CrossRef] [Google Scholar]
  15. Holmes, B., Owen, R. J., Steigerwalt, A. G. & Brenner, D. J.(1984).Flavobacterium gleum, a new species found in human clinical specimens. Int J Syst Bacteriol 34, 21–25.[CrossRef] [Google Scholar]
  16. Hugo, C. J., Segers, P., Hoste, B., Vancanneyt, M. & Kersters, K.(2003).Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53, 771–777.[CrossRef] [Google Scholar]
  17. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J.(2003).Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef] [Google Scholar]
  18. Kim, M. K., Im, W.-T., Shin, Y. K., Lim, J. H., Kim, S.-H., Lee, B. C., Park, M.-Y., Lee, K. Y. & Lee, S.-T.(2004).Kaistella koreensis gen. nov., sp. nov., a novel member of the Chryseobacterium–Bergeyella–Riemerella branch. Int J Syst Evol Microbiol 54, 2319–2324.[CrossRef] [Google Scholar]
  19. Kim, K. K., Kim, M. K., Lim, J. H., Park, H. Y. & Lee, S. T.(2005). Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol 55, 1287–1293.[CrossRef] [Google Scholar]
  20. Kim, K. K., Lee, K. C., Oh, H.-M. & Lee, J.-S.(2008).Chryseobacterium aquaticum sp. nov., isolated from a water reservoir. Int J Syst Evol Microbiol 58, 533–537.[CrossRef] [Google Scholar]
  21. Kumar, S., Tamura, K. & Nei, M.(2004).mega 3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  22. Lee, K., Lee, H. K., Choi, T.-H. & Cho, J.-C.(2007).Sejongia marina sp. nov., isolated from Antarctic seawater. Int J Syst Evol Microbiol 57, 2917–2921.[CrossRef] [Google Scholar]
  23. Lin, Y.-C., Uemori, K., de Briel, D. A., Arunpairojana, V. & Yokota, A.(2004).Zimmermannella helvola gen. nov. sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae. Int J Syst Evol Microbiol 54, 1669–1676.[CrossRef] [Google Scholar]
  24. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  25. NCCLS(2003).Performance standards for antimicrobial disk susceptibility tests, 8th edn. Approved Standard, M2-A8. Wayne, PA: National Committee for Clinical Laboratory Standards.
  26. O'Sullivan, L. A., Rinna, J., Humphreys, G., Weightman, A. J. & Fry, J. C.(2006). Culturable phylogenetic diversity of the phylum ‘Bacteroidetes’ from river epilithon and coastal water and description of novel members of the family Flavobacteriaceae: Epilithonimonas tenax gen. nov., sp. nov. and Persicivirga xylanidelens gen. nov., sp. nov. Int J Syst Evol Microbiol 56, 169–180.[CrossRef] [Google Scholar]
  27. Park, M. S., Jung, S. R., Lee, K. H., Lee, M.-S., Do, J. O., Kim, S. B. & Bae, K. S.(2006).Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., isolated from roots of sand-dune plants. Int J Syst Evol Microbiol 56, 433–438.[CrossRef] [Google Scholar]
  28. Park, S. C., Kim, M. S., Baik, K. S., Kim, E. M., Rhee, M. S. & Seong, C. N.(2008).Chryseobacterium aquifrigidense sp. nov., isolated from a water-cooling system. Int J Syst Evol Microbiol 58, 607–611.[CrossRef] [Google Scholar]
  29. Quan, Z.-X., Kim, K. K., Kim, M. K., Jin, L. & Lee, S.-T.(2007).Chryseobacterium caeni sp. nov., isolated from bioreactor sludge. Int J Syst Evol Microbiol 57, 141–145.[CrossRef] [Google Scholar]
  30. Skerman, V. B. D.(1967).A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  31. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Woods & N. R. Krieg. Washington, DC: American Society for Microbiology.
  32. Tai, C.-J., Kuo, H.-P., Lee, F.-L., Che, H.-K., Yokota, A. & Lo, C.-C.(2006).Chryseobacterium taiwanense sp. nov., isolated from soil in Taiwan. Int J Syst Evol Microbiol 56, 1771–1776.[CrossRef] [Google Scholar]
  33. Vancanneyt, M., Vandamme, P., Segers, P., Torck, U., Coopman, R., Kersters, K. & Hinz, K.-H.(1999).Riemerella columbina sp. nov., a bacterium associated with respiratory disease in pigeons. Int J Syst Bacteriol 49, 289–295.[CrossRef] [Google Scholar]
  34. Vandamme, P., Bernardet, J.-F., Segers, P., Kersters, K. & Holmes, B.(1994). New perspectives in the classification of the Flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44, 827–831.[CrossRef] [Google Scholar]
  35. Xie, C. H. & Yokota, A.(2003). Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef] [Google Scholar]
  36. Yi, H., Yoon, H. I. & Chun, J.(2005).Sejongia antarctica gen. nov., sp. nov. and Sejongia jeonii sp. nov., isolated from the Antarctic. Int J Syst Evol Microbiol 55, 409–416.[CrossRef] [Google Scholar]
  37. Yoon, J. H., Lee, M. H., Oh, T. K. & Park, Y. H.(2005).Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda. Int J Syst Evol Microbiol 55, 1015–1019.[CrossRef] [Google Scholar]
  38. Yoon, J.-H., Kang, S.-J., Jung, Y.-T. & Oh, T.-K.(2008).Muricauda lutimaris sp. nov., isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 58, 1603–1607.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.006619-0
Loading
Planobacterium taklimakanense gen. nov., sp. nov., a member of the family Flavobacteriaceae that exhibits swimming motility, isolated from desert soil
Int J Syst Evol Microbiol 59, 1672 (2009); https://doi.org/10.1099/ijs.0.006619-0
/content/journal/ijsem/10.1099/ijs.0.006619-0
/content/journal/ijsem/10.1099/ijs.0.006619-0
Loading

Data & Media loading...

Supplements

vol. , part 7, pp. 1672 - 1678

Actively motile cells of strain X-65 ( gen. nov., sp. nov.). [AVI file](9753 KB)

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error