RT Journal Article SR Electronic(1) A1 Lee, Soon DongYR 2009 T1 Amycolatopsis ultiminotia sp. nov., isolated from rhizosphere soil, and emended description of the genus Amycolatopsis JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 6 SP 1401 OP 1404 DO https://doi.org/10.1099/ijs.0.006577-0 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, designated strain RP-AC36T, was isolated from a cliff-associated plant (Peucedanum japonicum Thunb.) in the Republic of Korea and its taxonomic status was determined by using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequence analysis showed that the organism formed a distinct clade within the radiation of the genus Amycolatopsis. The chemotaxonomic properties supported the assignment of the isolate to the genus Amycolatopsis. High levels of 16S rRNA gene sequence similarity were found with Amycolatopsis sulphurea (98.2 %), Amycolatopsis halotolerans (97.5 %) and Amycolatopsis jejuensis (97.1 %). DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relative A. sulphurea. Based on the phenotypic and genotypic evidence, it is suggested that the organism be assigned as representing a novel species of the genus Amycolatopsis, for which the name Amycolatopsis ultiminotia sp. nov. is proposed. The type strain is RP-AC36T (=NRRL B-24662T=DSM 45180T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.006577-0