1887

Abstract

A novel Gram-positive-staining, aerobic, spore-forming, rod-shaped bacterium, designated strain YIM 91119, was isolated from Ebinur Lake in Xinjiang Province, north-west China. Cells were motile, produced terminal endospores and grew at pH 6.0–8.0 (optimally at pH 7.0), 4–45 °C (optimally at 28–37 °C) and 1–22 % (w/v) NaCl, (optimally at 10–15 %, w/v). Comparative 16S rRNA gene sequence analysis showed that strain YIM 91119 belongs to the genus , exhibiting the highest sequence similarity with respect to the type strain of (97.8 %); the next most similar 16S rRNA gene sequences were those of the type strains of (96.8 %), (96.5 %) and (95.8 %). DNA–DNA hybridization with AS 1.4250 showed a relatedness of 55 %. The major fatty acids of strain YIM 91119 were anteiso-C, iso-C and anteiso-C. The peptidoglycan type was A1 (directly cross-linked -diaminopimelic acid). The genomic DNA G+C content was 40.1 mol% and the predominant respiratory quinone was MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. On the basis of the evidence from this polyphasic study, strain YIM 91119 represents a novel species of the genus , for which the name sp. nov. is proposed, with YIM 91119 (=DSM 19802 =CCTCC AA 208015) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.006569-0
2009-07-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/7/1620.html?itemId=/content/journal/ijsem/10.1099/ijs.0.006569-0&mimeType=html&fmt=ahah

References

  1. Ahmed, I., Yokota, A. & Fujiwara, T.(2007).Gracilibacillus boraciitolerans sp. nov., a highly boron-tolerant and moderately halotolerant bacterium isolated from soil. Int J Syst Evol Microbiol 57, 796–802.[CrossRef] [Google Scholar]
  2. Carrasco, I. J., Márquez, M. C., Yanfen, X., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A.(2006).Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. Int J Syst Evol Microbiol 56, 599–604.[CrossRef] [Google Scholar]
  3. Cerny, G.(1978). Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 113–122.[CrossRef] [Google Scholar]
  4. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  5. Cui, H.-L., Tohty, D., Feng, J., Zhou, P.-J. & Liu, S.-J.(2006a).Natronorubrum aibiense sp. nov., an extremely halophilic archaeon isolated from Aibi salt lake in Xin-Jiang, China, and emended description of the genus Natronorubrum. Int J Syst Evol Microbiol 56, 1515–1517.[CrossRef] [Google Scholar]
  6. Cui, H.-L., Tohty, D., Zhou, P.-J. & Liu, S.-J.(2006b).Haloterrigena longa sp. nov. and Haloterrigena limicola sp. nov., extremely halophilic archaea isolated from a salt lake. Int J Syst Evol Microbiol 56, 1837–1840.[CrossRef] [Google Scholar]
  7. DeLong, E. F.(1992). Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89, 5685–5689.[CrossRef] [Google Scholar]
  8. Deutch, C. E.(1994). Characterization of a novel salt-tolerant Bacillus sp. from the nasal cavities of desert iguanas. FEMS Microbiol Lett 121, 55–60.[CrossRef] [Google Scholar]
  9. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  11. Felsenstein, J.(1985). Conference limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  12. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  13. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  14. Kroppenstedt, R. M.(1982). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2367.[CrossRef] [Google Scholar]
  15. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  16. Lawson, P. A., Deutch, C. E. & Collins, M. D.(1996). Phylogenetic characterization of a novel salt-tolerant Bacillus species: description of Bacillus dipsosauri sp. nov. J Appl Bacteriol 81, 109–112.[CrossRef] [Google Scholar]
  17. Leifson, E.(1960).Atlas of Bacterial Flagellation. London: Academic Press.
  18. Li, W.-J., Xu, P., Schumann, P., Zhang, Y.-Q., Pukall, R., Xu, L.-H., Stackebrandt, E. & Jiang, C.-L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef] [Google Scholar]
  19. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  20. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  21. Qian, Y.-B., Wu, Z.-N., Yang, Q., Zhang, L.-Y. & Wang, X.-Y.(2007). Ground-surface conditions of sand-dust event occurrences in the southern Junggar Basin of Xinjiang, China. J Arid Environ 70, 49–62.[CrossRef] [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  24. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  25. Smibert, R. M. & Krieg, N. R.(1981). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Philips. Washington, DC: American Society for Microbiology.
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  27. Wainø, M., Tindall, B. J., Schumann, P. & Ingvorsen, K.(1999).Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49, 821–831.[CrossRef] [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  29. Xin, H., Itoh, T., Zhou, P., Suzuki, K., Kamekura, M. & Nakase, T.(2000).Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50, 1297–1303.[CrossRef] [Google Scholar]
  30. Xu, P., Li, W.-J., Tang, S.-K., Zhang, Y.-Q., Chen, G.-Z., Chen, H.-H., Xu, L.-H. & Jiang, C.-L.(2005).Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55, 1149–1153.[CrossRef] [Google Scholar]
  31. Yang, Y., Cui, H.-L., Zhou, P.-J. & Liu, S.-J.(2007).Haloarcula amylolytica sp. nov., an extremely halophilic archaeon isolated from Aibi salt lake in Xin-Jiang, China. Int J Syst Evol Microbiol 57, 103–106.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.006569-0
Loading
/content/journal/ijsem/10.1099/ijs.0.006569-0
Loading

Data & Media loading...

Supplements

vol. , part 7, pp. 1620 - 1624

Polar lipid profile of strain YIM 91119 grown in 10 % modified ISP5 medium.

Cellular fatty acid compositions of strain YIM 91119 and XH-63 .

[PDF file of Supplementary Fig. S1 and Table S1](86 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error