1887

Abstract

Three strains of rod-shaped and motile lactic acid bacteria, NRIC 0736, NRIC 0735 and NRIC 0737 were isolated from sap obtained from two different oak trees ( sp.) on Awashima Island, Kagawa Prefecture, Japan. The three strains showed nearly identical 16S rRNA gene sequences (>99.7 % sequence similarity). The novel strains showed low 16S rRNA gene sequence similarities to recognized species of lactic acid bacteria. High gene sequence similarities were found between strain NRIC 0736 and NRIC 0604 (96.3 %), DSM 20444 (95.3 %) and DSM 20605 (95.3 %). DNA–DNA relatedness values revealed the genotypic separation of the three novel isolates from these recognized species. It is concluded that the three new isolates represent a novel species, for which the name sp. nov. is proposed. The type strain is NRIC 0736 (=JCM 15457=DSM 21376).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.006478-0
2009-11-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2662.html?itemId=/content/journal/ijsem/10.1099/ijs.0.006478-0&mimeType=html&fmt=ahah

References

  1. Brosius, J., Dull, T. J., Sleeter, D. D. & Noller, H. F. ( 1981; ). Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol 148, 107–127.[CrossRef]
    [Google Scholar]
  2. Carr, J. G. & Davies, A. ( 1970; ). Homofermentative lactobacilli of cider including Lactobacillus mali nov. spec. J Appl Bacteriol 33, 768–774.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Rodrigues, U. M., Ash, C., Aguirre, M., Farrow, J. A. E., Martinez-Murcia, A., Philips, B. A., Williams, A. M. & Wallbanks, S. ( 1991; ). Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA. FEMS Microbiol Lett 77, 5–12.[CrossRef]
    [Google Scholar]
  4. Endo, A. & Okada, S. ( 2005; ). Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials. Int J Syst Evol Microbiol 55, 83–85.[CrossRef]
    [Google Scholar]
  5. Ezaki, T., Yamamoto, N., Ninomiya, K., Suzuki, S. & Yabuuchi, E. ( 1983; ). Transfer of Peptococcus indolicus, Peptococcus asaccharolyticus, Peptococcus prevotii and Peptococcus magnus to the genus Peptostreptococcus and proposal of Peptostreptococcus tetradius sp. nov. Int J Syst Bacteriol 33, 683–698.[CrossRef]
    [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Hucker, G. J. & Conn, H. J. ( 1923; ). Method of Gram staining. N Y State Agric Exp Stn Tech Bull 93, 3–37.
    [Google Scholar]
  9. Kaneuchi, C., Seki, M. & Komagata, K. ( 1988; ). Taxonomic study of Lactobacillus mali Carr and Davis 1970 and related strains: validation of Lactobacillus mali Carr and Davis 1970 over Lactobacillus yamanashiensis Nonomura 1983. Int J Syst Bacteriol 38, 269–272.[CrossRef]
    [Google Scholar]
  10. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  11. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  12. Manome, A., Okada, S., Uchimura, T. & Komagata, K. ( 1998; ). The ratio of l-form to d-form of lactic acid as a criteria for the identification of lactic acid bacteria. J Gen Appl Microbiol 44, 371–374.[CrossRef]
    [Google Scholar]
  13. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  14. Nonomura, H. ( 1983; ). Lactobacillus yamanashiensis subsp. yamanashiensis and Lactobacillus yamanashiensis subsp. mali sp. and subsp. nov., nom. rev. Int J Syst Bacteriol 33, 406–407.[CrossRef]
    [Google Scholar]
  15. Okada, S., Hosoi, Y., Takahashi, M. & Kozaki, M. ( 1991; ). A rapid method for the determination of the fermentation types, homo- and hetero-fermentation, of lactic acid bacteria. Bull JFCC 7, 6–10 (in Japanese).
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  19. Toda, T. ( 1928; ). Flagellar staining. Nihon Ijishinpo 283, 113(in Japanese)
    [Google Scholar]
  20. Zhu, H., Qu, F. & Zhu, L. H. ( 1993; ). Isolation of genomic DNAs from plants, fungi, and bacteria using benzyl chloride. Nucleic Acids Res 21, 5279–5280.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.006478-0
Loading
/content/journal/ijsem/10.1099/ijs.0.006478-0
Loading

Data & Media loading...

Supplements

vol. , part 11, pp. 2662 - 2665

Cells of NRIC 0736 grown on an MRS slant at 30°C for 15 h showing peritrichous flagella.



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error