Three strains of rod-shaped and motile lactic acid bacteria, NRIC 0736, NRIC 0735 and NRIC 0737 were isolated from sap obtained from two different oak trees ( sp.) on Awashima Island, Kagawa Prefecture, Japan. The three strains showed nearly identical 16S rRNA gene sequences (>99.7 % sequence similarity). The novel strains showed low 16S rRNA gene sequence similarities to recognized species of lactic acid bacteria. High gene sequence similarities were found between strain NRIC 0736 and NRIC 0604 (96.3 %), DSM 20444 (95.3 %) and DSM 20605 (95.3 %). DNA–DNA relatedness values revealed the genotypic separation of the three novel isolates from these recognized species. It is concluded that the three new isolates represent a novel species, for which the name sp. nov. is proposed. The type strain is NRIC 0736 (=JCM 15457=DSM 21376).


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vol. , part 11, pp. 2662 - 2665

Cells of NRIC 0736 grown on an MRS slant at 30°C for 15 h showing peritrichous flagella.


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