Thirteen novel strains were isolated from root nodules of three different leguminous plants of the genera , and grown in China. Cells of these strains were Gram-negative, strictly aerobic, non-spore-forming, motile rods. Phylogenetic analysis of 16S rRNA gene sequences revealed that they belong to the genus . A representative strain, CCBAU 25010, showed 98.8 % 16S rRNA gene sequence similarity to its closest phylogenetic relative, IS123. The 16S–23S intergenic spacer (ITS) sequence of CCBAU 25010 shared 91.5 and 87.2 % sequence similarity, respectively, with those of CFN 42 and LMG 14904. Analysis of the sequences of the housekeeping genes and was in agreement with the results of ITS sequence analysis. The gene sequence of CCBAU 25010 was identical to that of CFN 299. DNA–DNA hybridization values for strain CCBAU 25010 ranged from 20.7 % (with USDA 1844) to 34.4 % (with USDA 2370). Cell protein SDS-PAGE, BOX-PCR and several phenotypic characteristics, such as use of sole carbon sources and antibiotic resistance, could differentiate the novel strains from defined species. We therefore propose that the novel strains reported in this study form a novel species, sp. nov., with the type strain CCBAU 25010 (=LMG 24135 =JCM 14777).


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vol. , part 8, pp. 1919 - 1923

SDS-PAGE protein profiles of representative novel strains and reference strains.

BOX-PCR fingerprints of representative novel strains and reference strains.

Neighbour-joining trees based on 16S–23S rRNA ITS sequences and partially congruent, concatenated and gene sequences of sp. nov. strains and phylogenetically related species of the genus .

Results of DNA–DNA hybridization analysis.

[PDF file of Supplementary Figures and Table](336 KB)

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